Agilent, 5190-6874, Agilent Bio SEC-5, 2000Å, 21.2 x 50 mm, 5 µm

Agilent Bio SEC-5, 2000Å, 21.2 x 50 mm, 5 µm. A preparative scale size exclusion column for the purification of very large biological analytes. Resolving range 150 kDa to >10 MDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 21.2 mm
LC Platform: Preparative HPLC
Length: 50 mm
Maximum Temperature: 80 °C
Molecular Weight Upper Limit: > 10,000,000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisMonoclonal Antibodies (mABs)PeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 2000 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115709
pH Range: 2-8.5

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The Agilent Bio SEC-5, 2000Å, 21.2 x 50 mm, 5 µm column offers reliable size exclusion chromatography for the analysis and purification of biomolecules, including proteins, mAbs, and large biomolecular complexes. With a 2000Å pore size and a 5 µm particle size, this column ensures excellent resolution and recovery for high-molecular-weight species. Its short 50 mm length and wide 21.2 mm internal diameter make it ideal for rapid separations and semi-preparative to preparative scale workflows in biopharmaceutical research, process development, and quality control.

This column is particularly suited for applications such as aggregate and fragment analysis, molecular weight determination, and sample desalting. Its robust silica-based stationary phase offers high chemical stability and reproducible performance across a wide pH range. The column is compatible with Agilent HPLC and UHPLC systems, including the 1260 Infinity II Bio-Inert and 1290 Infinity II Bio LC, as well as other major chromatography platforms designed for bioanalytical applications.

For method scalability, complementary Bio SEC-5 columns in various dimensions and pore sizes are available. This product streamlines workflow efficiency, supporting high-throughput laboratories and providing confidence in results critical to biotherapeutic development and routine analysis.