BD, 550296, BD Pharmingen™ Purified Mouse Anti-Rat CD4

Alternative Name: Cd4; CD4 antigen; p55; W3/25 antigen; T-cell surface glycoprotein CD4
Reactivity: Rat (QC Testing)
Isotype: Mouse BALB/c IgG2a, κ
Immunogen: Rat T-cell blasts
Application: Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Concentration: 62.5 µg/ml
RRID: AB_393591
Storage Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunohistochemistry: Clone OX-35 is recommended to test for immunohistochemical staining of acetone-fixed frozen sections of rat spleen and thymus. IHC of formalin-fixed paraffin embedded sections is not recommended.  This antibody has been reported to stain the CD4 subset of T lymphocytes. The isotype control recommended for use with this antibody is purified mouse IgG2a (Cat. No. 550339). For optimal indirect immunohistochemical staining, the OX-35 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with biotin conjugated rat anti-mouse IgG2a (Cat. No. 550332) as the secondary antibody and streptravidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880).