BD, 550881, BD Pharmingen™ PerCP-Cy™5.5 Rat Anti-Mouse IgM

Alternative Name: Ighm; Igh-M; Immunoglobulin M; Igh6; muH; immunoglobulin heavy constant mu
Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Immunogen: Pooled Mouse Ig
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
Recommended Assay Procedures: Recommended Assay Procedures PerCP-Cy™5.5 Rat Anti-Mouse IgM may be used as a primary or secondary reagent in immunofluorescent staining. For flow cytometric detection of intracytoplasmic IgM, we recommend FITC Rat Anti-Mouse IgM (Cat. No. 553437). PerCP-Cy5.5 tandem fluorochrome emission is collected in the Fluorescence-3 (FL3) channel of BD FACScan™ and BD FACSCalibur™ flow cytometry systems. For tandem conjugates incorporating PerCP (e.g., PerCP-Cy5.5), the excitation and emission properties of PerCP and the kinetics of energy exchange between the fluorochromes of the tandem dye may limit their effectiveness on high-speed and/or sorting flow cytometers.