BD, 551311, BD Pharmingen™ Purified Rat Anti-Human CD120b

Alternative Name: TNFRSF1B; p75 TNFR; p75 TNF Receptor; TNFR80; TNF-RII; TNF-R2
Reactivity: Human (QC Testing)
Isotype: Rat IgG2b, κ
Immunogen: COS-expressed recombinant human TNFRII
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
Workshop Number: V C006; VI BP200, C83
RRID: AB_394146
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The purified hTNFR-M1 (Cat. No. 551311) antibody can be used for the immunofluorescent staining (≤ 1 µg antibody/10e6 cells) and flow cytometric analysis of human nucleated cells to measure their expressed levels of surface TNFRII. An appropriate immunoglobulin isotype control is clone R35-38 (Cat. No. 555846). A three-layer staining protocol is recommended for maximizing the detection of TNFRII expressed by cells as detailed in the figure legend.Please note also that as a consequence of in vivo or in vitro activation, cell surface TNFRII can either be shed by cells or transiently expressed at higher levels. As a result, cellular activation can affect the cell's overall expressed level of surface TNFRII.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.