BD, 551454, BD Pharmingen™ Purified Mouse Anti-Human C1qRp (CD93)

Alternative Name: C1qRp
Reactivity: Human (QC Testing)
Isotype: Mouse IgM, κ
Immunogen: C1q-binding protein
Application: Flow cytometry (Routinely Tested), Immunoprecipitation, Western blot (Reported)
Concentration: 0.5 mg/ml
RRID: AB_394201
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Immunofluorescent Staining and Flow Cytometric Analysis: The staining technique and blocking controls are described in detail by C. Prussin and D. Metcalfe. A suitable mouse IgM, κ isotype control (Cat. No. 555581) for assessing the level of background staining on human cells is recommended: use at comparable concentrations to antibody of interest (eg, ≤ 0.5 µg Ab/1 million cells) (see Figure). For specific methodology, please visit the protocols section under "Multicolor Flow Cytometry" at http://www.bdbiosciences.com/us/s/resources. Western Blotting and immunoprecipitation: When run under non-reducing conditions, Human CD93 migrates as a 100 kDa protein; due to high level of glycosylation CD93 migrates as 126 kDa under reducing conditions. The R3 antibody is suitable to detect CD93 by Western blotting and immunprecipitation as described; however, the antibody is not tested for this application at BD Biosciences Pharmingen. Reactivity of the antibody with the reduced protein is dramatically decreased. Modulation of monocyte phagocytic activity: Pretreatment of monocytes with R3 neutralizes CD93-mediated enhancement of phagocytosis. In addition, when immobilized, R3 mimics CD93- MBL- or SPA-mediated enhancement of phagocytosis; however, the antibody is not tested for this application at BD Biosciences Pharmingen.