BD, 551457, BD Pharmingen™ Purified NA/LE Mouse Anti-Human CD43

Alternative Name: Leukosialin; LSN; Galactoglycoprotein; GALGP; GPL115; SPN; Sialophorin
Reactivity: Human (QC Testing)
Isotype: Mouse IgG1, κ
Immunogen: Activated Human T Cells
Application: Flow cytometry (Routinely Tested), Functional assay, Immunohistochemistry, Immunoprecipitation, Western blot (Reported)
Concentration: 1.0 mg/ml
Workshop Number: IV N601; V AS297
RRID: AB_394204
Storage Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.