BD, 553174, BD Pharmingen™ APC Hamster Anti-Mouse TCR β Chain

Reactivity: Mouse (QC Testing)
Isotype: Armenian Hamster IgG2, λ1
Immunogen: TCR affinity-purified from mouse T-cell hybridoma DO-11.10
Application: Flow cytometry (Routinely Tested)
Concentration: 0.2 mg/ml
RRID: AB_398534
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures It has been observed that pre-incubation of thymus cell suspensions at 37°C for 2 to 4 hours prior to staining enhances the ability of anti-CD3e and anti-TCR β chain mAbs to detect the T cell receptor on immature thymocytes. The APC fluorochrome is excited by laser lines from 595 to 647 nm, and its emission is collected in a detector for fluorescence wavelengths between 640 and 680 nm.