BD, 553738, BD Pharmingen™ Purified Rat Anti-Mouse CD14

Reactivity: Mouse (QC Testing)
Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
Immunogen: Recombinant Mouse CD14
Application: Flow cytometry (Routinely Tested), Western blot (Reported)
Concentration: 0.5 mg/ml
RRID: AB_395020
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Caution: Our studies demonstrate that Mouse BD Fc Block™ anti-CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142) and antibodies of rat IgG2b isotype may interfere with the reactivity of rmC5-3 antibody in a concentration-dependent manner. For inhibition of non-specific reactivity, we recommend use of purified mouse IgG at 10-100-fold excess. Other reported applications include western blot analysis. For in vitro and in vivo blocking of LPS binding to CD14, we recommend the NA/LE™ format of the alternate anti-mouse CD14 mAb 4C1/CD14 (Cat. No. 557896).  Use of rmC5-3 antibody for immunohistochemical staining has been reported; however, we have been unable to reproduce those results at BD Biosciences Pharmingen.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.