Product Description
Alternative Name: IgG1/IgG2a; Ig gamma-1/2a; Immunoglobulin gamma 1/2a
Reactivity: Rat (QC Testing), Mouse (Tested in Development)
Isotype: Mouse IgG2a
Immunogen: Pooled Rat IgG
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_395110
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Recommended Assay Procedures: Recommended Assay Procedures FITC Mouse Anti-Rat IgG1/2a may be used as a secondary reagent in immunofluorescent staining.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. An isotype control should be used at the same concentration as the antibody of interest. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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