BD, 554546, BD Pharmingen™ Biotin Rat Anti-Human IL-6

Reactivity: Human (QC Testing)
Isotype: Rat IgG2a
Immunogen: Recombinant human IL-6
Application: ELISA Detection (Routinely Tested), Neutralization, Western blot (Reported)
Concentration: 0.5 mg/ml
RRID: AB_395470
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures: Recommended Assay Procedures ELISA Detection: The biotinylated MQ2-39C3 antibody (Cat. No. 554546) is useful as a detection antibody for a sandwich ELISA for measuring human IL-6 protein levels. Biotinylated MQ2-39C3 antibody can be paired with the purified MQ2-13A5 (Cat. No. 554543) as the capture antibody, with recombinant human IL-6 (Cat. No. 550071) as the standard. Biotinylated MQ2-39C3 antibody should be titrated (0.5 - 2.0 µg/ml) to determine optimal concentration for ELISA detection in this system. To obtain linear standard curves, doubling dilutions of human IL-6 ranging from ~15 to 2000 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our website, www.bdbiosciences.com, and go to the protocols section or the chapter on ELISA in the Immune Function Handbook. Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines tested (e.g., mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23, SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF). Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems not serum or plasma samples. For testing human IL-6 in complex biological fluids such as serum or plasma, our human IL-6 BD OptEIA™ ELISA Set (Cat. No. 555220) and BD OptEIA™ ELISA Kit II (Cat. No. 550799) are recommended. Western Blot: The MQ2-39C3 antibody has been reported to be useful for Western blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.