BD, 554599, BD Pharmingen™ Recombinant Mouse IL-13

Preparation And Storage: Preparation And Storage Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. Rapidly thaw and quick-spin product prior to use. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, prepare stock solutions diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 5 μg/ml for long term storage. Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
Recommended Assay Procedures: Recommended Assay Procedures Biological Activity Activity Assay: Measured by proliferation using  TF-1 indicator cells Specific Activity Range :  0.6 - 5 x 10^7 Units*/mg ED50 : 0.2 - 1.5 ng/ml ; Observed dose response relationship: 50 fold * Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation .