BD, 555642, BD Pharmingen™ Purified Mouse Anti-Human CD123

Alternative Name: IL3RA; IL-3RA; IL-3Rα; IL-3R-alpha; Interleukin-3 receptor subunit alpha
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: Human IL-3Rα Transfected Cell Line
Application: Flow cytometry (Routinely Tested), Immunofluorescence, Western blot (Reported)
Concentration: 0.5 mg/ml
Workshop Number: VI C-67
RRID: AB_395999
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures This antibody is useful for western blotting and for immunofluorescent labelling of cells for fluorescence microscopy or flow cytometry. For flow cytometric applications, a three-step labelling procedure is recommended for amplifying signal. For example: 1. Incubate (~1 million) cells with ~2 µg of antibody for 45-60 minutes at 4° C. Wash three times in PBS/0.1% sodium azide/1.0% heat -inactivated fetal bovine serum (PBS-FBS). 2. Incubate cells with ~0.5 µg biotinylated goat anti-mouse IgS (Cat. No. 553999) at 4° C for 30 minutes. Wash three times. 3. Incubate cells with ~0.1 µg streptavidin-PE (Cat. No. 554061) at 4° C for 30 minutes. Wash three times. Resuspend in 0.5 ml of PBS-FBS and analyze by flow cytometry.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.