BD, 557818, BD Phosflow™ Alexa Fluor® 488 Mouse Anti-ZAP70 (pY319)/Syk (Y352)

Alternative Name: ZAP-70; SRK; STD; TZK; Zeta-chain associated protein kinase, 70kD
Reactivity: Human (QC Testing), Mouse, Rat (Tested in Development)
Isotype: Mouse IgG1
Immunogen: Human phosphorylated ZAP70 Peptide
Application: Intracellular staining (flow cytometry) (Routinely Tested)
Target Molecular Weight: 70 kDa
Vol. Per Test: 20 µl
RRID: AB_396885
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
Recommended Assay Procedures: Recommended Assay Procedures Jurkat cells treated with H2O2 are suggested as a positive control. However, other cell types or methods may also be used for detection of phosphorylated ZAP70.
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC). For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. All other brands are trademarks of their respective owners.