BD, 562260, BD Pharmingen™ PE Mouse Anti-Human LAP

Alternative Name: Latency-associated peptide; TGFB1; TGFbeta; Transforming growth factor beta
Reactivity: Human (QC Testing)
Isotype: Mouse BALB/c IgG1, κ
Immunogen: Human TGF-β1
Application: Intracellular staining (flow cytometry) (Routinely Tested), Flow cytometry (Tested During Development)
Vol. Per Test: 5 µl
RRID: AB_10895568
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Recommended Assay Procedures: Recommended Assay Procedures Suggested Staining Procedures for PE Mouse anti-Human LAP Antibody: 1.  Harvest the PBMCs after stimulation (24 hours) with plate-bound Purified NA/LE Mouse Anti-Human CD3 (Cat. No. 555329) and Purified NA/LE Mouse Anti-Human CD28 (Cat. No. 555725). 2.  Wash the cells twice with stain buffer (eg. BD Pharmingen™ Stain Buffer (FBS), Cat. No. 554656). 3.  Stain 1 × 10^6 cells with PerCP Mouse anti-Human CD4 (Cat. No. 347324) and either with the PE Mouse anti-Human LAP antibody (Cat. No. 562260) or with PE Mouse IgG1, κ Isotype control (Cat. No. 555749) for 30 minutes on ice, protected from light. 4.  Wash cells twice with stain buffer. 5.  Stain for Alexa Fluor® 488 Mouse anti-Human FoxP3, refer to Technical Data Sheet of Cat. No. 560047 for detailed protocol. In brief, a.  Add 2 ml of 1 × FoxP3 buffer A to the cell pellet. b.  Centrifuge and incubate in 0.5 ml of buffer C for 30 minutes. c.  Wash cells twice with stain buffer and stain with anti-FoxP3 antibody for 30-45min. d.  Wash cells twice with stain buffer and acquire on the Flow cyotmeter.