BD Pharmingen™ Human BD Fc Block™ 564220

Reactivity: Human (QC Testing), Rhesus (Tested in Development)
Isotype: Human IgG1
Application: Flow cytometry (Routinely Tested)
Concentration: 0.5 mg/ml
RRID: AB_2728082
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C.
Recommended Assay Procedures: Recommended Assay Procedures Incubate 1 million cells suspended in 50-100 µL of staining buffer with 2.5 µg of Human BD Fc Block™ (10 minutes at room temperature) followed by staining with the desired fluorescent antibody. No washing step is needed between the blocking and staining steps.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).