BD, 564703, BD Pharmingen™ Purified Rat Anti-Blimp-1

Alternative Name: BLIMP-1; BLIMP1; PRDM1; PR domain containing 1, with ZNF domain; PRDI-BF1
Reactivity: Human (QC Testing), Mouse (Tested in Development)
Isotype: Rat IgG2a, κ
Immunogen: Mouse Blimp-1 Recombinant Protein
Application: Intracellular staining (flow cytometry) (Routinely Tested), Immunohistochemistry-paraffin, Western blot (Tested During Development)
Concentration: 0.5 mg/ml
RRID: AB_2738902
Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Note: Staining with PE Rat Anti-Blimp-1 antibody (Cat. No. 564702) is recommended for the flow cytometric analysis of Blimp-1 expression in human peripheral blood plasma cells.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.