BD Pharmingen™ PE Goat Anti-Human Ig κ Light Chain 567014

Reactivity: Human (QC Testing)
Isotype: Goat IgG, F(ab')2
Immunogen: Human Ig κ Light Chains
Application: Flow cytometry (Routinely Tested)
Vol. Per Test: 5 µl
RRID: AB_2870005
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography.
Recommended Assay Procedures: Recommended Assay Procedures Some B lymphocytes and non-B leucocytes express Fc Receptors that can selectively bind various immunoglobulins from the plasma. As a result, these "cytophilic antibodies" can be detected on the cell surface using anti-Ig antibodies. Overnight incubation of peripheral blood mononuclear cells (PBMC) at 37ºC in complete tissue culture medium reportedly reduces the amount of these cytophilic antibodies observed on the PBMC. Costaining the PBMC with anti-CD20 or anti-CD19 and gating on the B lymphocytes eliminates detection of the cytophilic antibodies on the non-B cell populations, but a small proportion of the gated B lymphocytes may still express cytophilic antibodies. BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD Comp beads are appropriate for your specific cellular application.
Product Notices: Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). An isotype control should be used at the same concentration as the antibody of interest. Source of all serum proteins is from USDA inspected abattoirs located in the United States. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. Please refer to http://regdocs.bd.com to access safety data sheets (SDS). Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.