BD, 611880, BD Transduction Laboratories™ Purified Mouse Anti-Synaptophysin

Reactivity: Rat (QC Testing), Human, Mouse (Tested in Development)
Isotype: Mouse IgG1
Immunogen: Rat Synaptophysin aa. 205-306
Application: Western blot (Routinely Tested), Bioimaging, Immunofluorescence (Tested During Development)
Target Molecular Weight: 38 kDa
Concentration: 250 µg/ml
RRID: AB_399360
Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
Regulatory Status: RUO
Preparation And Storage: Preparation And Storage Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices: Product Notices Since applications vary, each investigator should titrate the reagent to obtain optimal results. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. Source of all serum proteins is from USDA inspected abattoirs located in the United States. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR. Triton is a trademark of the Dow Chemical Company. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.