Bio-Rad, 1725035, iScript™ gDNA Clear cDNA Synthesis Kit, 100 x 20 µl reactions

100 x 20 µl reactions, 400 µl 5x supermix, contains reverse transcriptase, RNase inhibitor, dNTPs, primers, MgCl 2 , stabilizers; 400 µl no-RT control supermix; 50 µl DNase; 150 µl DNase buffer
Overview
Use iScript gDNA Clear cDNA Synthesis Kit for easy and rapid generation of qPCR-ready cDNA free of genomic DNA (gDNA) for accurate gene expression analysis by real-time PCR.
Features and Benefits
• Effective gDNA removal in purified RNA samples
• Proprietary DNase buffer for maintaining RNA integrity during gDNA removal
• Convenient all-in-one, single-tube RT supermix
• Ready-to-use no-RT control supermix for monitoring gDNA contamination
• gDNA-free cDNA ready in 36 min
gDNA-Free cDNA Ready in 36 Minutes
This kit allows you to effectively remove contaminating gDNA and reliably synthesize cDNA with minimal hands-on time in an easy-to-follow protocol, resulting in cDNA free of genomic DNA in 36 minutes.
Packaging Options
Supporting Documentation
Download the iScript gDNA Clear cDNA Synthesis Kit Product Insert for detailed information about step-by-step protocols for cDNA synthesis and troubleshooting tips.
Related Products
• cDNA Synthesis Kits
• Real-Time PCR Supermixes and Kits
• PrimePCR™ PCR Primers & Assays
Related Categories
• PCR and qPCR Reagents
• Real-Time PCR Detection Systems
• PCR Plastic Consumables
• Instrument Validation Tools and Services