Biolegend, 100210, Alexa Fluor® 488 anti-mouse CD3 Antibody, 100μg

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: γδTCR-positive T-T hybridoma D1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested IHC-F, 3D IHC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Application References(PubMed link indicates BioLegend citation): Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Axelrod ML, et al. 2022. Nature. 611:818. PubMed Kang YA, et al. 2023. J Exp Med. 220:. PubMed Arora S, et al. 2023. PLoS One. 18:e0286211. PubMed Cautivo KM, et al. 2022. Immunity. 55:254. PubMed Jang HJ, et al. 2022. Cancer Res. 82:2593. PubMed Ariyasu Y, et al. 2022. Int J Mol Sci. 23: . PubMed Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed Liu G, et al. 2023. Commun Biol. 6:102. PubMed Vijver SV, et al. 2023. Front Immunol. 13:1100730. PubMed Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed Feist M, et al. 2021. Cancer Gene Ther. 28:98. PubMed Yeh CH, et al. 2022. Immunity. 55:272. PubMed Schneider C, et al. 2018. Cell. 174:271. PubMed Wu J, et al. 2022. Nat Commun. 13:7321. PubMed Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed Okajima T, et al. 2021. Front Immunol. 12:738041. PubMed Bieber M, et al. 2021. Int J Mol Sci. 22:. PubMed Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed Dauer Née Joppe K, et al. 2021. J Neurochem. 159:554. PubMed Upadhyaya P, et al. 2021. J Immunother Cancer. 9:00. PubMed Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed Kodumudi KN, et al. 2019. Front Immunol. 10:1939. PubMed Logan C, Bowen C, and Menko A 2017. Sci Rep. . 10.1038/s41598-017-16456-5. PubMed Brandt S, et al. 2020. J Am Soc Nephrol. 31:2589. PubMed Park JJ, et al. 2021. Nat Commun. 12:1222. PubMed Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed Wang C, et al. 2021. Cell Stem Cell. . PubMed Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed Lau CH, et al. 2022. Antioxidants (Basel). 11:. PubMed Wan W, et al. 2013. Cardiovasc Res. 97:580. PubMed Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed Amor C, et al. 2020. Nature. 583:127. PubMed Wei SC, et al. 2020. Cancer Discov. . PubMed Mills C, et al. 2022. Cells. 11:. PubMed Wong HS, et al. 2021. Cell. . PubMed Xiao S, et al. 2007. J Exp Med. 204:1691. PubMed Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed Hsieh C, et al. 2014. J Immunol. 193:3693. PubMed Counoupas C, et al. 2020. NPJ Vaccines. 0.28125. PubMed Wang B, et al. 2021. J Immunother Cancer. 9:. PubMed Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed Pietronigro E, et al. 2019. Sci Rep. 9:12055. PubMed Hanihara-Tatsuzawa F, et al. 2014. J Biol Chem. 389:30925. PubMed Broderick L, et al. 2019. Nat Commun. 10:3644. PubMed Karandikar SH, et al. 2019. Anal Chem. 91:3405. PubMed Riding AM, et al. 2022. iScience. 25:104660. PubMed Langhauser F, et al. 2014. Stroke. 45:1799. PubMed Karandikar SH, et al. 2019. JCI Insight. 5. PubMed Wang S, et al. 2022. J Inflamm Res. 14:7107. PubMed Li MY, et al. 2021. Developmental Cell. 56:2547. PubMed MaruYama T, et al. 2015. J Leukoc Biol. 98: 385-393. PubMed Cheng Q, et al. 2018. PLoS Pathog. 14:e1007440. PubMed Liu Y, et al. 2019. Sci Rep. 9:18970. PubMed Zhao L,et al. 2017. Nat Commun. . 10.1038/s41467-017-01181-4. PubMed Xu JL, et al. 2022. Front Pharmacol. 13:911663. PubMed Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed Lee-Sayer SSM, et al. 2018. Eur J Immunol. 48:803. PubMed Zhou P, et al. 2022. Mol Ther Oncolytics. 25:236. PubMed Kuhn NF et al. 2019. Cancer cell. 35(3):473-488 . PubMed Hsing CH, et al. 2021. Biomedicines. 9:. PubMed
RRID: AB_493530 (BioLegend Cat. No. 100212) AB_389301 (BioLegend Cat. No. 100210)
Structure: Ig superfamily, CD3/TCR, 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC-complex
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.
Gene ID: 12502
UniProt: View information about CD3 on UniProt.org
Clone: 17A2
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Rat IgG2b, κ