Biolegend, 100703, Biotin anti-mouse CD8a Antibody, 50μg

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality testedIHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
Application References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP) Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete) Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP) Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block) Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC) Shih FF, et al. 2006. J. Immunol. 176:3438. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete) Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed Lee CH, et al. 2009. Clin. Cancer Res. PubMed Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed Guo Y, et al. 2008. Blood 112:480. PubMed Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed Kenna TJ, et al. 2008. Blood 111:2091. PubMed Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Medyouf H, et al. 2010. Blood 115:1175. PubMed Riedl P, et al. 2009. J. Immunol. 183:370. PubMed Apte SH, et al. 2010. J. Immunol. 185:998. PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Cui L, et al. 2015. J Control Release. 206:220. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed McGee MC, et al. 2022. Bio Protoc. 12:. PubMed Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed Miyake K, et al. 2023. Nat Commun. 14:2694. PubMed Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed Gargaro M, et al. 2022. Immunity. 55:1032. PubMed Marié IJ, et al. 2022. Bio Protoc. 12:e4438. PubMed Tomiaki C, et al. 2022. Front Immunol. 13:1014462. PubMed Giannou AD, et al. 2023. Immunity. 56:125. PubMed Khaw YM, et al. 2023. EMBO Rep. 24:e54228. PubMed Gonçalves R, et al. 2023. iScience. 26:105972. PubMed Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed De Giovanni M, et al. 2022. Cell. 185:815. PubMed Friedman D, et al. 2022. J Immunol. 208:1845. PubMed McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed Mendes A, et al. 2021. Life Sci Alliance. 4:00. PubMed Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed Elsner R, et al. 2015. PLoS Pathog. 10: 1004976. PubMed Wang L, et al. 2019. Cell Rep. 29:1848. PubMed Zhu C, et al. 2018. Sci Rep. 5.113194444. PubMed Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed Guo F, et al. 2022. Front Immunol. 13:833100. PubMed Wilfahrt D, et al. 2021. Elife. 10:. PubMed Zhou L, et al. 2021. Cell Mol Immunol. 18:698. PubMed Donley D, et al. 2016. PLoS One. 11: 0162404. PubMed Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed Gravano D, et al. 2010. PLoS One. 5:e13528. PubMed Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed Iturri L, et al. 2021. Immunity. . PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Hutter K, et al. 2020. Molecular Cell. 78(5):876-889.e6. PubMed Yang X, et al. 2020. Blood Sci. 2:89. PubMed Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed Van de Velde L, et al. 2016. Cell Rep. 17:2247-2258. PubMed Xu X, et al. 2020. J Cell Biol. 219:00:00. PubMed Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed Mann M, et al. 2018. Cell Rep. 25:2992. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Zhang C, et al. 2020. Cell Metabolism. 31(1):148-161.e5.. PubMed Suah AN, et al. 2021. J Clin Invest. 131:. PubMed Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed Köchl R, et al. 2020. Elife. 9:00. PubMed Pearce V, et al. 2015. J Immunol. 195: 3206 - 3217. PubMed Yu VWC, et al. 2017. Cell. 168:944. PubMed Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed Matsumura T et al. 2019. Cell Rep. 27(2):561-571 . PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Shen Z, et al. 2021. iScience. 24:103014. PubMed Dong S, et al. 2021. Nature. 591:117. PubMed Progatzky F, et al. 2021. Nature. 599:125. PubMed Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed Onodera T, et al. 2021. Immunity. 54:2385. PubMed Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed Kaya B, et al. 2020. Cell Reports. 32(5):107979. PubMed Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed Chen Z, et al. 2019. J Exp Med. 216:152. PubMed Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed Aftabizadeh M, et al. 2021. JCI Insight. 6:. PubMed Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed Morikawa M, et al. 2016. PLoS One. 11:e0163607. PubMed Holm J, et al. 2015. PLoS One. 10:125495. PubMed Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Zeis P, et al. 2020. Immunity. 53:775. PubMed Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed Macal M et al. 2018. Immunity. 48(4):730-744 . PubMed Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed Ji Y, et al. 2019. Nat Immunol. 1.303472222. PubMed Wu J, et al. 2021. STAR Protoc. 2:101022. PubMed Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed Lam KC, et al. 2021. Cell. 184:5338. PubMed Roderick J, et al. 2014. Proc Natl Acad Sci U S A. 111:14436. PubMed He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed Bajaña S, et al. 2022. iScience. 25:103732. PubMed Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed Yoshida H, et al. 2019. Cell. 176:897. PubMed Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed Rai S, et al. 2022. Nat Commun. 13:5346. PubMed Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed Luo H, et al. 2019. Cell Rep. 26:945. PubMed Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed Zheng C, et al. 2022. Acta Pharm Sin B. 12:3726. PubMed Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed Young K, et al. 2021. Cell Stem Cell. . PubMed Davidson M, et al. 2013. PLoS One. 8:76258. PubMed Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
RRID: AB_312742 (BioLegend Cat. No. 100703) AB_312743 (BioLegend Cat. No. 100704)
Structure: Ig superfamily, CD8α chain, 34 kD
Distribution: Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells
Function: Co-receptor for TCR
Ligand/Receptor: MHC class I molecule
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Zamoyska R. 1994. Immunity 1:243. 3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.
Gene ID: 12525
UniProt: View information about CD8alpha on UniProt.org
Clone: 53-6.7
Regulatory Status: RUO
Other Names: T8, Lyt2, Ly-2
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.