Biolegend, 100801, Purified anti-mouse CD8a Antibody, 50μg

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Concanavalin A-stimulated BALB/c splenic T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedICC - VerifiedIHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: partial inhibition of T-cell responses to IL-2 and cytotoxic induction of splenic T cells1, immunohistochemistry of acetone-fixed frozen sections2, and immunofluorescence. The 5H10-1 antibody competes with 53-6.7 antibody (Cat. No. 100702) for binding to thymocytes1.Clone 5H10-1 is not recommended for immunohistochemistry of formalin-fixed paraffin sections.
Application References(PubMed link indicates BioLegend citation): Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. Frei K, et al. 1997. J. Exp. Med. 185:2177.
Product Citations: Helfen A, et al. 2022. Neoplasia. 28:100792. PubMed Han C, et al. 2021. Cell Reports. 34(6):108706. PubMed Hu J, et al. 2019. Mol Ther Nucleic Acids. 16:650. PubMed Scutts SR, et al. 2018. Cell Rep. 25:1953. PubMed
RRID: AB_312762 (BioLegend Cat. No. 100801) AB_312763 (BioLegend Cat. No. 100802)
Structure: Ig superfamily, CD8α chain, 34 kD
Distribution: Most thymocytes, T cell subset, some NK cells
Function: Co-receptor for TCR
Ligand/Receptor: MHC class I molecule
Cell Type: Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Zamoyska R. 1994. Immunity 1:243. 3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.
Gene ID: 12525
UniProt: View information about CD8alpha on UniProt.org
Clone: 5H10-1
Regulatory Status: RUO
Other Names: T8, Lyt2, Ly-2
Isotype: Rat (SD) IgG2b, λ