Biolegend, 100804, FITC anti-mouse CD8a Antibody, 500μg

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Concanavalin A-stimulated BALB/c splenic T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: partial inhibition of T-cell responses to IL-2 and cytotoxic induction of splenic T cells1, and immunohistochemistry2 of acetone-fixed frozen sections. The 5H10-1 antibody competes with 53-6.7 antibody (Cat. No. 100702) for binding to thymocytes1.Clone 5H10-1 is not recommended for immunohistochemistry of formalin-fixed paraffin sections.
Application References(PubMed link indicates BioLegend citation): Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. Frei K, et al. 1997. J. Exp. Med. 185:2177. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed Nakajima A, et al. 2014. PLoS One. 9:105904. PubMed
Product Citations: Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed Gonçalves R, et al. 2023. iScience. 26:105972. PubMed McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed Bhatt K, et al. 2018. mSphere. 3:e00352. PubMed Shrivastava T, et al. 2021. Front Immunol. 12:641447. PubMed Shin JE, et al. 2021. Heliyon. 7:e08433. PubMed Nakajima A, et al. 2014. PLoS One. 9:105904. PubMed Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed Wang F, et al. 2019. MAbs. 12:1685350. PubMed Korrer M, Routes J 2014. PLoS One. 9:91370. PubMed Moutuou MM, et al. 2020. Methods Mol Biol. 2111:267. PubMed Derada Troletti C, et al. 2021. Cell Reports. 35(9):109201. PubMed Cá B, et al. 2019. Front Microbiol. 1.876388889. PubMed Wu J, et al. 2021. STAR Protoc. 2:101022. PubMed Johannsen A, et al. 2010. J Immunol. 185:3445. PubMed Suhail A, et al. 2019. Cell Rep. 29:3522. PubMed
RRID: AB_312764 (BioLegend Cat. No. 100803) AB_312765 (BioLegend Cat. No. 100804)
Structure: Ig superfamily, CD8α chain, 34 kD
Distribution: Most thymocytes, T cell subset, some NK cells
Function: Co-receptor for TCR
Ligand/Receptor: MHC class I molecule
Cell Type: Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Zamoyska R. 1994. Immunity 1:243. 3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.
Gene ID: 12525
UniProt: View information about CD8alpha on UniProt.org
Clone: 5H10-1
Regulatory Status: RUO
Other Names: T8, Lyt2, Ly-2
Isotype: Rat (SD) IgG2b, λ