Biolegend, 101301, Purified anti-mouse CD16/32 Antibody, 50μg

CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Sorted pre-B cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 93 can be used for blocking of CD16/CD32 interactions with the Fc domain of immunoglobulins, but is not the same clone as 2.4G2. The 93 mAb is specific to the common epitope of CD16/CD32. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and blocking of Fc-mediated reactions in functional studies2,4,23. It is useful for blocking non-specific binding of immunoglobulin to Fc receptors. For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with purified anti-CD16/CD32 antibody (=1.0 µg per 106 cells in 100 µL volume) for 5-10 minutes on ice prior to immunostaining. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101330) (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered).
Application References(PubMed link indicates BioLegend citation): Personal communication (IP) Oliver AM, et al. 1999. Hybridoma 18:113. (Block) Brummel R and Lenert P. 2005. J. Immunol. 174:2429. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (Block) Clements JL, et al. 2006. J. Immunol. 177:905. Mohamed W, et al. 2010. Infect Immun. 78:3306. PubMed Ouchi T, et al. 2011. J. Exp Med. 208:2607. PubMed Kmieciak M, et al. 2011. J. Vis. Exp. 47:2381. PubMed Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed Koon HW, et al. 2013. J. Vis. Exp. 68:4208. PubMed Hegde VL, et al. 2013. J Biol Chem. 288:36810. PubMed Huang J, et al. 2013. J. Immunol Methods. 387:254. PubMed Dutow P, et al. 2014. J Infect Dis. PubMed Fan Y, et al. 2014. J Exp Med. 211:313. PubMed Huang HN, et al. 2014. Antimicrob Agents Chemother. 58:1538. PubMed Takei S, et al. 2014. Vaccine. 32:3066. PubMed Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Cekanaviciute E, et al. 2014. J Immunol. 193:139. PubMed Kimura T, et al. 2014. Int Immunol. 26:697. PubMed Everad A, et al. 2014. Nat Commun. 5:5648. PubMed Cenci E, et al. 2006. J. Leuko. Biol. 79(1):40-5. (Block)
Product Citations: Lee J, et al. 2015. Hum Reprod. 10.1093/humrep/dev222. PubMed Bussières-Marmen S, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.72. PubMed Chida J, et al. 2020. PLoS Pathog. 16:e1008823. PubMed Iyer S, et al. 2021. Cancer Discov. 0.725. PubMed Park E, et al. 2021. Journal of Neuroscience Research. . PubMed Gabitova-Cornell L, et al. 2020. Cancer Cell. 38(4):567-583.e11. PubMed Kosti P, et al. 2021. Cell Reports Medicine. 2(4):100227. PubMed Barkaway A, et al. 2021. Immunity. . PubMed Sade-Feldman M,et al. 2017. Nat Commun.. 10.1038/s41467-017-01062-w. PubMed Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed Gan T, et al. 2019. Front Microbiol. 9:3174. PubMed Furuhashi K, et al. 2017. Immunology. 151:227. PubMed Fomin ME, et al. 2017. Cell Med. 0.45625. PubMed Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed Filippini D, et al. 2019. Sci Rep. 9:12286. PubMed Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed Zhang H, et al. 2022. Nat Cancer. 3:808. PubMed Fang Y, et al. 2022. Sci Immunol. 7:eabp9962. PubMed Koch MS, et al. 2022. Mol Ther Oncolytics. 26:275. PubMed Harapas CR, et al. 2022. Sci Immunol. 7:eabi4611. PubMed Ding P, et al. 2022. Elife. 11:. PubMed Deák P, et al. 2022. Cell Rep. 41:111563. PubMed Zhong J, et al. 2022. J Adv Res. 41:145. PubMed Melo-Silva CR, et al. 2022. Cell Rep. 41:111676. PubMed Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed Wu Q, et al. 2023. Front Immunol. 13:1081719. PubMed Li L, et al. 2023. iScience. 26:106095. PubMed Torrente D, et al. 2023. J Neuroinflammation. 20:56. PubMed Bai X, et al. 2023. Nat Commun. 14:1247. PubMed Rocca Y, et al. 2023. Methods Mol Biol. 2618:187. PubMed Dang YZ, et al. 2023. Neoplasma. 70:123. PubMed Senatus L, et al. 2023. Commun Biol. 6:280. PubMed Kudo T, et al. 2023. iScience. 26:106342. PubMed Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed Zhang YN, et al. 2023. Nat Commun. 14:1985. PubMed Iguchi A, et al. 2023. iScience. 26:106375. PubMed Feng Y, et al. 2023. J Transl Med. 21:290. PubMed Ji Y, et al. 2023. Nat Cell Biol. 25:726. PubMed Liu JC, et al. 2023. Int J Mol Sci. 24:. PubMed Petenkova A, et al. 2023. Nat Commun. 14:2761. PubMed Xu J, et al. 2023. Discov Oncol. 14:68. PubMed An R, et al. 2022. Cell Insight. 1:100047. PubMed Wang K, et al. 2023. Nat Commun. 14:2950. PubMed Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed Li M, et al. 2023. BMC Cancer. 23:497. PubMed Mawambo G, et al. 2023. J Neuroinflammation. 20:145. PubMed Akasaka H, et al. 2023. JCI Insight. 8:. PubMed Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Foster A, et al. 2021. EBioMedicine. 71:103571. PubMed Kiepura A, et al. 2021. Int J Mol Sci. 22:. PubMed Rajagopalan A, et al. 2021. Cell Rep Methods. 1:. PubMed Guo H, et al. 2020. Curr Protoc Immunol. 131:e107. PubMed Van Braeckel-Budimir N, et al. 2021. J Immunother Cancer. 9:. PubMed Wang Z, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed Xiang G, et al. 2022. Cell Death Dis. 13:451. PubMed Nederlof R, et al. 2022. Front Immunol. 13:908023. PubMed Cerrato G, et al. 2021. Cell Death Differ. 28:1733. PubMed Da Mesquita S, et al. 2021. Nature. 593:255. PubMed Jebbawi F, et al. 2021. Parasite Immunol. 43:e12834. PubMed Thomas R, et al. 2021. Immunology. 163:478. PubMed Herz J, et al. 2021. Neuron. 109:3609. PubMed Li H, et al. 2022. Cancer Cell. 40:36. PubMed Glynn RA, et al. 2022. J Immunol. 208:371. PubMed Kozuki S, et al. 2022. Genes Cells. 27:192. PubMed Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed Guttman O, et al. 2022. J Cell Biol. 221: . PubMed Miyao T, et al. 2022. Elife. 11: . PubMed Yin K, et al. 2022. Genes Dev. 36:533. PubMed Malhi H, et al. 2022. Cell Rep Med. 3:100658. PubMed Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed Sugimoto S, et al. 2022. Nat Metab. 4:775. PubMed Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed Que W, et al. 2022. Sci Adv. 8:eabo4413. PubMed Li X, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:1077. PubMed Akalu YT, et al. 2022. Elife. 11: . PubMed Zhang X, et al. 2022. EBioMedicine. 84:104275. PubMed Bottermann K, et al. 2022. Basic Res Cardiol. 117:48. PubMed Hao J, et al. 2022. Cell Rep. 41:111804. PubMed McNamara NB, et al. 2023. Nature. 613:120. PubMed Miró L, et al. 2022. Cancers (Basel). 14: . PubMed Liu P, et al. 2023. Clin Immunol. 246:109212. PubMed Ullah I, et al. 2023. Cell Rep Med. 4:100893. PubMed Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed Cousin N, et al. 2022. Cells. 12: . PubMed Peng Q, et al. 2023. EMBO Rep. 24:e56034. PubMed Tichet M, et al. 2023. Immunity. 56:162. PubMed Zhou H, et al. 2023. Front Immunol. 13:1059687. PubMed Mandour MF, et al. 2023. Front Med (Lausanne). 9:1057252. PubMed Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed Novaj A, et al. 2023. Int J Mol Sci. 24: . PubMed Ishikawa T, et al. 2023. Bio Protoc. 13:e4588. PubMed Liu X, et al. 2022. Pain. 163:508. PubMed Zhang M, et al. 2022. Mol Imaging Biol. 24:280. PubMed Zhang W, et al. 2022. Dev Cell. 57:329. PubMed Kodali S, et al. 2022. J Immunol. 208:1085. PubMed Chen K, et al. 2022. Adv Sci (Weinh). 9:e2104106. PubMed Liu C, et al. 2022. Nat Nanotechnol. 17:531. PubMed Correa S, et al. 2020. ACS Nano. 14:2224. PubMed Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed Benitez AA, et al. 2020. Commun Biol. 0.371527778. PubMed Castoldi Â, et al. 2020. Nat Commun. 3.310416667. PubMed Mevel R, et al. 2020. eLife. 9:00. PubMed Fan Y, et al. 2014. J Exp Med. 211:313. PubMed Berry CT, et al. 2020. Cell Reports. 31(2):107474.. PubMed Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed Biljes D, et al. 2017. EXCLI J. 0.86875. PubMed Zhang R, et al. 2019. Cell Rep. 28:2647. PubMed McGill BE et al. 2018. The Journal of Neuroscience. 38(1):200-219 . PubMed Zou B, et al. 2017. PLoS Pathogens. 13(6):e1006436. PubMed Kitagawa S, et al. 2022. NPJ Vaccines. 7:115. PubMed Golden GJ, et al. 2021. MBio. 12:e0118121. PubMed Yu Y, et al. 2022. iScience. 25:105004. PubMed Huang Y, et al. 2021. Nat Immunol. 22:586. PubMed MacLean M, et al. 2022. Commun Biol. 5:177. PubMed Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed Weng L, et al. 2016. Sci Rep. 6:38632. PubMed Browning D, et al. 2017. Gene Ther. 10.1038/gt.2016.88. PubMed Morris MG, et al. 2020. Comp Med. 70:336. PubMed Sano T, et al. 2021. Cell Reports. 36:109608. PubMed Tan H,et al. 2017. Mucosal Immunol. . 10.1038/mi.2017.89. PubMed Clarke F,et al. 2017. PLoS One. 10.1371/journal.pone.0186625. PubMed Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed Minutti CM, et al. 2019. Immunity. 50:645. PubMed Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed Takeda Y, et al. 2018. Innate Immun. 24:323. PubMed Goel S, et al. 2017. Nature. 548:471. PubMed Matsui S, et al. 2018. Am J Pathol. 188:2059. PubMed Bhatia D, et al. 2019. JCI Insight. 4:e132826. PubMed Noh JR, et al. 2018. Sci Rep. 8:15222. PubMed Flores M, et al. 2008. FASEB J. 3.459027778. PubMed Tong J, et al. 2011. J Immunol. 186:3686. PubMed Martos-Rus C, et al. 2021. Sci Rep. 11:2974. PubMed Liu P, et al. 2021. Front Med (Lausanne). 8:625130. PubMed Song J, et al. 2021. J Am Heart Assoc. 10:e017329. PubMed Jiang W, et al. 2021. Oncol Lett. 22:625. PubMed Khamissi FZ, et al. 2022. Sci Adv. 8:eabm5900. PubMed Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed Feng J, et al. 2021. iScience. 24:103004. PubMed Wu L, et al. 2022. Front Immunol. 13:860761. PubMed Bauer KM, et al. 2022. JCI Insight. 7:. PubMed Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed Clark AL, et al. 2021. Diabetes Obesity and Metabolism. :. PubMed Stahnke S, et al. 2021. Current Biology. 31(10):2051-2064.e8. PubMed Yang SB, et al. 2021. Cancers (Basel). 13:00. PubMed Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed Azuma M, et al. 2012. Oncoimmunology. 1:581. PubMed Yamazaki S, et al. 2012. PLoS One. 7:e51665. PubMed Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed Hays C, et al. 2020. eLife. 8:e48772.. PubMed Li Y, et al. 2020. Cell Rep. 30:1753. PubMed Gawden‐Bone CM et al. 2018. Immunity. 49(3):427-437 . PubMed Tyagi AM et al. 2018. Immunity. 49(6):1116-1131 . PubMed Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed Diao L, et al. 2022. iScience. 25:105511. PubMed Zhang Y, et al. 2022. Cancers (Basel). 14:. PubMed Mirshahi F, et al. 2022. Cell Rep. 38:110454. PubMed Sun SJ, et al. 2022. Commun Biol. 5:867. PubMed Chen X, et al. 2021. Cell Death Differ. 28:1237. PubMed Roberto Raúl SG, et al. 2022. Breast Cancer (Auckl). 16:11782234211065154. PubMed Matthias J, et al. 2020. J Clin Invest. 130:4587. PubMed Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed Liang F, et al. 2021. Front Immunol. 12:658715. PubMed Guan H, et al. 2012. PLoS One. 7:e35650. PubMed Hazama D, et al. 2020. Cell Chemical Biology. 27(9):1181-1191.e7. PubMed Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed Godbersen-Palmer C, et al. 2020. J Immunol. 204:2973. PubMed Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed Mann M, et al. 2018. Cell Rep. 25:2992. PubMed Fiala GJ, et al. 2019. J Immunol. 203:569. PubMed Dave K et al. 2017. eLife. 6 pii: e23382. PubMed Kitashima DY, et al. 2018. EBioMedicine. 27:293. PubMed Li Y, et al. 2018. J Immunol. 201:2910. PubMed Carlson P, et al. 2019. Nat Cell Biol. 21:238. PubMed Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed Yang C, et al. 2020. J Control Release. 326:324. PubMed Liu D, et al. 2021. JCI Insight. 6:. PubMed Armaka M, et al. 2022. Genome Med. 14:78. PubMed Hoffman D, et al. 2021. Immunity. 54:2712. PubMed Shen H, et al. 2022. Nat Commun. 13:6030. PubMed Haugh KA, et al. 2021. eLife. 0.416666666666667. PubMed Tal MC, et al. 2020. MBio. 11:00. PubMed Zhang X, et al. 2020. J Immunol. 205:1743. PubMed Kovacs L, et al. 2020. PLoS One. 15:e0240455. PubMed Vackova J, et al. 2020. Int J Mol Sci. 21:00. PubMed Terrazas LI, et al. 2005. Int J Parasit. 35:1349. PubMed Bhattacherjee A, et al. 2019. Commun Biol. 2:450. PubMed Lieschke S, et al. 2019. Cells. 0.420833333. PubMed Sereni L, et al. 2018. J Allergy Clin Immunol. 142:1272. PubMed Fransen F, et al. 2017. Front Immunol. 1.295138889. PubMed Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed Wlodarska M et al. 2017. Cell host & microbe. 22(1):25-37 . PubMed Wang Y, et al. 2022. Front Immunol. 13:767630. PubMed Ma J, et al. 2021. Aging (Albany NY). 13:7211. PubMed Yu M, et al. 2021. J Clin Invest. 131:. PubMed Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed Cheng XS, et al. 2020. J Cell Mol Med. 24:8391. PubMed Kohn M, et al. 2021. Front Immunol. 580594:12. PubMed Liang Z, et al. 2020. Pain. 161:2089. PubMed Wang L, et al. 2017. Front Immunol.. 10.3389/fimmu.2017.01754. PubMed Fiaturi N, et al. 2018. J Cell Commun Signal. 314:L127. PubMed Barajas‐Mora EM et al. 2018. Molecular cell. 73(1):48-60 . PubMed Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed Koliaraki V et al. 2019. Cell reports. 26(3):536-545 . PubMed Finn J et al. 2019. Cell reports. 26(11):2942-2954 . PubMed Barger SR, et al. 2019. Nat Commun. 10:1249. PubMed Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed Caronni N, et al. 2018. Cancer Res. 78:1685. PubMed Weiner AI, et al. 2019. NPJ Regen Med. 4:17. PubMed Russell SL, et al. 2019. Cell Rep. 29:3564. PubMed Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed Chen C, et al. 2019. Cell Rep. 29:4200. PubMed Damle SR, et al. 2018. Allergy. 73:125. PubMed Gui J, et al. 2020. Nat Cancer. 1:603. PubMed Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed Sheller-Miller S, et al. 2021. Sci Adv. 7:. PubMed Mao W, et al. 2021. Int J Mol Sci. 22:. PubMed Dang Y, et al. 2022. Cancer Cell Int. 22:88. PubMed Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed Cong M, et al. 2021. Methods Mol Biol. 2388:59. PubMed Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed Escudero-Lara A, et al. 2022. Front Behav Neurosci. 16:946975. PubMed Flommersfeld S, et al. 2021. Immunity. :. PubMed Wong HS, et al. 2021. Cell. . PubMed Kusuyama J, et al. 2021. Cell Metabolism. 33(5):939-956.e8. PubMed Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed Gereke M, et al. 2012. J Vis Exp. 70: 4322. PubMed MacConmara M, et al. 2011. J Leukoc Biol. 89:137. PubMed Cassidy LD, et al. 2018. Autophagy. 1.455555556. PubMed Fransen F, et al. 2017. Front Immunol. 8:154. PubMed Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed McLaughlin PA et al. 2019. PLoS Pathog. 15(7):e1007847 . PubMed Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed Go MJ, et al. 2018. Mol Med Rep. 13:e0194423. PubMed McGrath-Morrow S, et al. 2017. Cytokine. 10.1016/j.cyto.2017.06.002. PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Ruf B, et al. 2021. Cancer Immunol Res. 9:1024. PubMed Muñoz NM, et al. 2022. Sci Rep. 12:14449. PubMed Hu G, et al. 2022. Cell Death Dis. 13:640. PubMed Sun Y, et al. 2022. Nat Commun. 13:3916. PubMed Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed Everard A, et al. 2014. Nat Commun. 5:5648. PubMed Francis M, et al. 2017. Toxicol Sci. 155(1):182-195. PubMed Hu J, et al. 2020. Theranostics. 7.154166667. PubMed Baasch S, et al. 2021. Cell. . PubMed Sutherland TE, et al. 2021. Frontiers in Immunology. 12:715209. PubMed Zheng J, et al. 2020. bioRxiv. . PubMed Csóka B, et al. 2018. JCI Insight. 38:200. PubMed Proto JD et al. 2018. Immunity. 49(4):666-677 . PubMed Rashidi M, et al. 2019. J Immunol. 203:736. PubMed Deng M, et al. 2020. Nat Commun. 11:2193. PubMed Hashimoto M, et al. 2019. FASEB J. 33:8069. PubMed Imani J, et al. 2021. JCI Insight. 6:. PubMed Dang Y, et al. 2021. J Cancer. 12:7158. PubMed Du Y, et al. 2022. Nat Commun. 13:231. PubMed Pishesha N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed Chin AL, et al. 2021. Nat Commun. 12:5138. PubMed Hong CH, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:925. PubMed Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed Hubbard JJ, et al. 2020. J Exp Med. :217. PubMed Xu S, et al. 2021. Immunity. . PubMed Tacconi C, et al. 2021. Cell Reports. 35(2):108993. PubMed Dieterich LC, et al. 2020. Angiogenesis. 1.24375. PubMed Duvigneau S, et al. 2016. Sci Rep. 6:37045. PubMed Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed Moon H, et al. 2020. Nat Commun. 4.270138889. PubMed Kulkarni U, et al. 2019. Mucosal Immunol. 12:545. PubMed Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed Qi S et al. 2016. eLife. 5 pii: e14756. PubMed Roy K, et al. 2019. Immunity. 50:616. PubMed Lin C, et al. 2022. Nat Commun. 13:6869. PubMed De Santi C, et al. 2022. Mol Ther Nucleic Acids. 29:643. PubMed Pereira M, et al. 2022. Cell Rep. 40:111225. PubMed Kim S, et al. 2022. Redox Biol. 54:102347. PubMed Chen Y, et al. 2022. Front Immunol. 13:835690. PubMed Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed Welte T, et al. 2021. iScience. 24:103349. PubMed Baier FA, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:745. PubMed Li Y, et al. 2021. Sci Transl Med. 13:. PubMed Dutow P, et al. 2014. J Infect Dis. 209:1269. PubMed Huang H, et al. 2014. Antimicrob Agents Chemother. 58:1538. PubMed Lehmann GL, et al. 2020. J Exp Med. 217:00:00. PubMed Zhao L, et al. 2020. EBioMedicine. 60:103020. PubMed Riedelberger M, et al. 2020. Bio Protoc. e3552:10. PubMed Matsumoto K, et al. 2017. PLoS One. 12(6):e0175406. PubMed Sauma D, et al. 2017. Immunol Res. 10.1007/s12026-017-8936-9. PubMed Gil-Pulido J, et al. 2017. PLoS One.. 10.1371/journal.pone.0181947. PubMed Yu L, et al. 2019. Elife. 8:e46677. PubMed Chen JC, et al. 2018. Front Immunol. 0.665277778. PubMed Peng L, et al. 2019. Mol Med Rep. 19:2876. PubMed Shin J, et al. 2018. Diabetes. 67:1068. PubMed Yan W, et al. 2021. Front Med (Lausanne). 8:752223. PubMed Sun C, et al. 2022. J Adv Res. 35:71. PubMed Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed Sundararaj K, et al. 2021. Immunology. 162:418. PubMed Jeong M, et al. 2022. JCI Insight. 7:. PubMed Zheng J, et al. 2020. Nature. . PubMed Unudurthi SD, et al. 2020. Life Sci. 117440:247. PubMed Yamakawa D, et al. 2021. Cell Reports. 34(10):108817. PubMed Troutman TD, et al. 2021. STAR Protocols. 2(1):100363. PubMed Li X, et al. 2020. Nat Commun. 4.877777778. PubMed Siggs O, et al. 2016. Proc Natl Acad Sci U S A. 113: 3706 - 3715. PubMed Seki T, et al. 2015. J Biochem. 158: 485 - 495. PubMed Cekanaviciute E, et al. 2014. J Immunol. 193:139. PubMed Hegde V, et al. 2013. J Biol Chem. 288:36810. PubMed Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed Chu A, et al. 2021. J Reprod Immunol. 145:103309. PubMed Frost JN, et al. 2021. Med (N Y). 2:164. PubMed Moon S, et al. 2021. J Exp Med. 218:. PubMed Hu CF, et al. 2021. Front Immunol. 12:638381. PubMed Kmieciak M, et al. 2011. J Vis Exp. 47: 2381. PubMed Huang BR, et al. 2020. Int J Mol Sci. 21:00. PubMed Lindborg JA, et al. 2021. Cell Reports. 34(9):108777. PubMed Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed Enomoto D, et al. 2017. PLoS One.. 10.1371/journal.pone.0183584. PubMed Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed Zeng J, et al. 2018. J Immunol. 200:3407. PubMed Hsu BE et al. 2019. Cell Rep. 27(13):3902-3915 . PubMed Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed Rebecca S Dickinson et al. 2017. Wellcome open research. 0.155555556 . PubMed Li B, et al. 2019. Oncol Rep. 41:608. PubMed Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed Fujimoto N, et al. 2020. PLoS Biol. 18:e3000704. PubMed Akk A, et al. 2019. Mol Immunol. 114:629. PubMed Tuong ZK, et al. 2021. iScience. 24:103326. PubMed Yu H, et al. 2021. Front Oncol. 11:736882. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Blanchard L, et al. 2022. STAR Protoc. 3:101444. PubMed Babagana M, et al. 2021. Aging (Albany NY). 13:19207. PubMed Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed Vilcaes AA, et al. 2020. Int J Mol Sci. :21. PubMed Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed Lehmann ML, et al. 2020. Brain Behav Immun. 88:735. PubMed Xu G, et al. 2020. Cell Rep. 32:108170. PubMed Takei S, et al. 2014. Vaccine. 32:3066. PubMed Constantinides M, et al. 2011. J Immunol. 187:309. PubMed Lenert R 2005. J Immunol. 174:2429. PubMed Ramachandran P, et al. 2019. Nature. 575:512. PubMed Spranger S, et al. 2017. Cancer Cell. 31:711. PubMed Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed Sanchez M et al. 2017. Cell host & microbe. 22(4):543-551 . PubMed Shibamoto M, et al. 2019. Int Heart J. 60:944. PubMed Matsuda T, et al. 2022. iScience. 25:105324. PubMed Takahashi F, et al. 2022. iScience. 25:104278. PubMed Saveljeva S, et al. 2022. Cell Metab. 34:106. PubMed Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed Shibuya M, et al. 2021. iScience. 24:103131. PubMed Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed Liu P, et al. 2020. Mol Cell. 748:77. PubMed Zhang Y, et al. 2020. Cancer Res. 80:3345. PubMed Okuma A, et al. 2017. Nat Commun.. 10.1038/s41467-017-02281-x. PubMed Kang YH, et al. 2019. Nat Commun. 10:912. PubMed Tellechea M, et al. 2018. J Immunol. 200:305. PubMed Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed Lyle C, et al. 2019. Sci Rep. 9:20257. PubMed Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed Mandour MF, et al. 2020. Infect Agent Cancer. 15:30. PubMed Kolter J, et al. 2020. Immunity. 50(6):1482-1497. PubMed Nakav S, et al. 2009. Nephrol Dial Transplant. 25:931. PubMed Miranda-Hernandez S, et al. 2011. J Immunol. 187:791. PubMed Bi J, et al. 2022. Front Microbiol. 13:927122. PubMed Zhang MH, et al. 2020. Front Immunol. 11:560. PubMed Tomimatsu M, et al. 2022. Sci Rep. 12:16656. PubMed López-Sanz C, et al. 2022. STAR Protoc. 3:101755. PubMed Saxena M, et al. 2020. Cancer Res. 80:3631. PubMed Chen C, et al. 2020. Cell Rep. 2136:30. PubMed Ouchi T, et al. 2011. J Exp Med. 208:2607. PubMed Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed Forster R, et al. 2019. FASEB J. 33:11210. PubMed Heinen A, et al. 2019. Mol Ther. 27:46:00. PubMed Simmons S et al. 2019. Elife. 8 pii: e41239. PubMed William M, et al. 2018. J Immunol. 200:4102. PubMed Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed Miura Y et al. 2018. eLife. 7 pii: e36572. PubMed Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed Alshetaiwi H, et al. 2020. Methods Mol Biol. 2236:177. PubMed Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed Lee SA, et al. 2021. Cells. 10:. PubMed Chida K, et al. 2021. Biol Pharm Bull. 44:1551. PubMed Kimura T, et al. 2014. Int Immunol. 26:697. PubMed Howe C, et al. 2016. Sci Rep. 6:28699. PubMed Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed Senatus L, et al. 2020. JCI Insight. 5:00. PubMed Matsumoto M, et al. 2021. Cell Host Microbe. 29(6):930-940.e4. PubMed Jin HS, et al. 2020. Cancer Immunol Res. 8:912. PubMed Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed Freeman SA et al. 2018. Cell. 172(1-2):305-317 . PubMed Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed Knox T, et al. 2019. Sci Rep. 9:6136. PubMed Rojo R, et al. 2019. Nat Commun. 10:3215. PubMed Schaftenaar FH, et al. 2019. Sci Rep. 9:17391. PubMed Deng M, et al. 2019. J Exp Med. 216:2838. PubMed Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed Clements J, et al. 2006. J Immunol. 177:905. PubMed Sakurai Y, et al. 2021. Pharmaceutics. 13:. PubMed Banskota S, et al. 2021. Inflamm Bowel Dis. 27:914. PubMed Magaletta ME, et al. 2022. Nat Commun. 13:457. PubMed Tzeng TT, et al. 2022. NPJ Vaccines. 7:60. PubMed Qiu Z, et al. 2020. Cancer Genet. 245:6. PubMed Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed Bonavita E, et al. 2020. Immunity. 1215:53. PubMed Luo X, et al. 2021. Neuron. 109:2691. PubMed McDonald MM, et al. 2021. Cell. 184(5):1330-1347.e13. PubMed Alam MM, et al. 2021. eneuro. 8(1):. PubMed Sitapara RA, et al. 2020. Mol Med. 26:63. PubMed Kim K, et al. 2015. Infect Immun . 83:1150. PubMed Penzo M, et al. 2010. J Immunol. 184:4497. PubMed Mehrpouya-Bahrami P, et al. 2019. J Biol Chem. 294:7669. PubMed Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed Blaszczak AM, et al. 2019. J Immunol. 202:2451. PubMed Sivaram N, et al. 2019. J Clin Invest. 130. PubMed Litwinoff EMS, et al. 2017. Obes Res Clin Pract. 12:174. PubMed Burl RB et al. 2018. Cell metabolism. 28(2):300-309 . PubMed Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed Dong H, et al. 2022. Nat Metab. 4:90. PubMed Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed Melero I, et al. 2015. Cancer Res. 75:497. PubMed Hegde V, et al. 2015. J Immunol. 194:5211. PubMed Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed Lyons DO, et al. 2018. PLoS One. 13:e0207704. PubMed Li A, et al. 2018. Cancer Lett. 431:54. PubMed Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed Phadke MS, et al. 2021. Cancer Immunol Res. 9:554. PubMed Sibler E, et al. 2021. Cells. 10:. PubMed Qin J, et al. 2020. Cancer Immunol Res. 8:1426. PubMed Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed Zhang M, et al. 2022. iScience. 25:104490. PubMed Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed Sibler E, et al. 2022. Cancers (Basel). 14:. PubMed Effern M, et al. 2020. Immunity. 53(3):564-580.e9. PubMed Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed Richardson M, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed Huanga J, et al. 2013. J Immunol Methods. 387:254. PubMed Malinczak CA, et al. 2019. J Immunol. 10.4049/jimmunol.1900741. PubMed Wang X, et al. 2018. Front Immunol. 9:2615. PubMed Kabir AU, et al. 2018. JCI Insight. 3. PubMed Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed
RRID: AB_312800 (BioLegend Cat. No. 101301) AB_312801 (BioLegend Cat. No. 101302)
Structure: Ig superfamily, 40-60 kD
Distribution: B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells
Function: Low affinity receptors for IgG
Ligand/Receptor: IgG
Cell Type: B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Unkeless JC. 1989. J. Clin. Invest. 83:355. 3. Qiu WQ, et al. 1990. Science 248:732.
Gene ID: 1413014131
UniProt: View information about CD16/32 on UniProt.org
Clone: 93
Regulatory Status: RUO
Other Names: Fcγ R III/II, Ly-17
Isotype: Rat IgG2a, λ