Biolegend, 102715, Alexa Fluor® 647 anti-mouse CD38 Antibody, 25μg

CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscle, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells, natural killer (NK) lymphocytes, myeloblasts, and erythroblasts. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse bone marrow pre-B cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality testedIHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Product Citations: Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Enterina JR, et al. 2022. Cell Rep. 38:110512. PubMed Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed Tan AHY, et al. 2019. Front Immunol. 10:1351. PubMed Nabar NR, et al. 2022. Autophagy. 18:204. PubMed Ray A, et al. 2015. PLoS One. 10: 0137314. PubMed Zhang Y, et al. 2018. J Exp Med. 215:1227. PubMed Lindhorst A, et al. 2021. Cell Death Dis. 12:579. PubMed Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
RRID: AB_2073334 (BioLegend Cat. No. 102716)
Structure: ADP-ribosyl hydrolase, 42 kD
Distribution: B cells, NK cells, T cell subset, brain, muscle, kidney
Function: Regulation of cellular activation/proliferation, adhesion, metabolism of cADPR and NAADP
Ligand/Receptor: CD31, hyaluronic acid
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Shubinsky G, et al. 1997. Immunity 7:315. 3. Cesano A, et al. 1998. J. Immunol. 160:1106. 4. Cockayne DA, et al. 1998. Blood 92:1324.
Gene ID: 12494
UniProt: View information about CD38 on UniProt.org
Clone: 90
Regulatory Status: RUO
Other Names: T10
Isotype: Rat IgG2a, κ