Biolegend, 103706, PE anti-mouse CD49d Antibody, 200μg

CD49d is a 150 kD glycoprotein, also known as α 4 integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α 4 β 1 ) or LPAM-1 (integrin α 4 β 7 ) complexes. CD49d plays a critical role in both adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin. The 9C10(MFR4.B) antibody, in combination with the R1-2 monoclonal antibody, completely blocks VCAM-1 binding to VLA-4.
200μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: (C57BL/6 x A/J)F1 mouse fetal liver mas
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of cell-cell adhesion and T cell costimulation1,2, and immunohistochemistry3 of acetone-fixed frozen sections. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103709 & 103710).
Application References(PubMed link indicates BioLegend citation): Kinashi T, et al. 1994. Blood Cells 20:25. (Block) Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block) Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) Jia W, et al. 2005. Blood 106:3854. (FC)
Product Citations: Valanparambil RM, et al. 2017. PLoS Pathog. 13:e1006647. PubMed Chung KJ, et al. 2023. Int J Mol Sci. 24:. PubMed Weng L, et al. 2016. Sci Rep. 6:38632. PubMed Zhao B, et al. 2021. Cell Reports. 36(3):109401. PubMed Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed Di C, et al. 2015. J Biol Chem. 290:12523. PubMed Uceda-Castro R, et al. 2022. Cell Rep Med. 3:100821. PubMed Simonetti G, et al. 2013. J Exp Med. 210:2887. PubMed Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed Tran NL, et al. 2022. Elife. 11:. PubMed
RRID: AB_313046 (BioLegend Cat. No. 103705) AB_313047 (BioLegend Cat. No. 103706)
Structure: Integrin family, associates with integrin β1 or β7, 150 kD
Distribution: T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells
Function: T cell costimulation, cell-cell and cell-matrix adhesion
Ligand/Receptor: VCAM-1, MAdCAM-1, fibronectin
Cell Type: B cells, Basophils, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Lobb RR, et al. 1994. J. Clin. Invest. 94:1722. 3. Berlin C, et al. 1993. Cell 74:185. 4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.
Gene ID: 16401
UniProt: View information about CD49d on UniProt.org
Clone: 9C10 (MFR4.B)
Regulatory Status: RUO
Other Names: α4 integrin, VLA-4 α chain, integrin α4, ITGA4
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.