Biolegend, 104320, PerCP/Cyanine5.5 anti-mouse/rat CD61 Antibody, 100μg

CD61 is a 110 kD integrin β chain also known as β 3 integrin or gpIIIa. It associates with the integrin α v chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin α IIb chain (CD41) to form the gpIIb/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of α v /β 3 integrin or vitronectin receptor to CD31 (PECAM-1).
100μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Vitronectin receptor protein from the mouse T-cell hybridoma 2B4.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 104325 and 104326).
Application References(PubMed link indicates BioLegend citation): Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block) Piali L, et al. 1995. J. Cell Biol. 130:451. (Block) Ashkar S, et al. 2000. Science 287:860. (Block) Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block) Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ) Carlson TR, et al. 2008.135:2193. PubMed Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed
RRID: AB_2715821 (BioLegend Cat. No. 104319) AB_2715822 (BioLegend Cat. No. 104320)
Structure: Integrin family, 110 kD
Distribution: Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells
Function: Attachment to matrix
Ligand/Receptor: Fibrinogen, von Willebrand factor, fibronectin
Cell Type: B cells, Endothelial cells, Macrophages, Mast cells, Megakaryocytes, Monocytes, Osteoclasts, Platelets, T cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. 2. Phillips DR, et al. 1991. Cell. 65:359. 3. Felding-Habermann B, et al. 1993. Curr Opinion Cell Biol. 5:864.
Gene ID: 1641629302
UniProt: View information about CD61 on UniProt.org
Clone: 2C9.G2 (HMβ3-1)
Regulatory Status: RUO
Other Names: β3 integrin, Integrin β3 chain, gpIIIa
Isotype: Armenian Hamster IgG
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.