Biolegend, 104804, PE anti-mouse CD33 Antibody, 100μg

CD33, also known as Siglec-3, Sigle, gp67, p67, and gp6, is a member of the sialic acid-binding immunoglobulin lectins (Siglec). CD33 extracellular portion contains two immunoglobulin domains (one IgV and one IgC2 domain). The intracellular portion of CD33 contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that are implicated in inhibition of cellular activity. CD33 is primarily expressed on granulocytes in peripheral blood and spleen, as well as on myeloid precursors in the bone marrow.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: RBL1 Transfected with full length mCD33
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2927954 (BioLegend Cat. No. 104803) AB_2927954 (BioLegend Cat. No. 104804)
Structure: Extracellular portion contains two immunoglobulin domains (IgV and IgC2 domain), intracellular portion contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs)
Distribution: Granulocytes in peripheral blood and spleen, myeloid precursors in the bone marrow
Function: Inhibition of cellular activity
Ligand/Receptor: Molecule with sialic acid residues
Cell Type: Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: Brinkman-Van der Linden, Els C M, et al. 2003. Mol Cell Biol. 23:4199-206.
Gene ID: 12489
UniProt: View information about CD33 on UniProt.org
Clone: W18124D
Regulatory Status: RUO
Other Names: sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67
Isotype: Rat IgG2a, λ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.