Biolegend, 104911, PerCP/Cyanine5.5 anti-mouse/rat CD81 Antibody, 25μg

CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1. CD81 is expressed on T and B cells, NK cells, dendritic cells, thymocytes, endothelial cells, and fibroblasts. CD81 induces B cell adhesion via the VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8 and CD82 in T cells.
25μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: CD81+ mouse B lymphoma 38C13
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The Eat-2 antibody reacts with mouse and rat CD81. Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, induction of homotypic adhesion of B lymphocytes1, stimulation of B cells undergo early apoptotic events1, and promotion of T cell motility2.
Application References(PubMed link indicates BioLegend citation): 1. Maecker HT, et al. 2000. Hybridoma 19:15. (IP, WB, Stim) 2. Clark KL, et al. 2001. J. Immunol. 167:5115. (IP, WB, Activ) 3. Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200. PubMed 4. Castro-Seoane R, et al. 2012. PLoS Pathog. 8:1002538. PubMed
Product Citations: Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
RRID: AB_2562994 (BioLegend Cat. No. 104911) AB_2562995 (BioLegend Cat. No. 104912)
Structure: TM4SF tetraspanin, 26 kD
Distribution: T cells and B cells, NK cells, dendritic cells, thymocytes, endothelial cells, fibroblasts
Function: Regulates cell growth
Cell Type: B cells, Dendritic cells, Endothelial cells, Fibroblasts, NK cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Levy S, et al. 1998. Annu. Rev. Immunol. 16:89. 3. Maeker HT, et al. 1997. FASEB J. 11:428. 4. Boismenu R, et al. 1996. Science. 271:198.
Gene ID: 1252025621
UniProt: View information about CD81 on UniProt.org
Clone: Eat-2
Regulatory Status: RUO
Other Names: TAPA-1
Isotype: Armenian Hamster IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.