Biolegend, 105402, Purified anti-mouse/human Helios Recombinant Antibody, 100μg

Helios is a member of the Ikaros family of zinc finger transcription factors. It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members. Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes. It is found at high levels in a subpopulation of regulatory T cells. Helios plays an important role in T cell development and homeostasis. Overexpression of Helios profoundly alters αβ T cell differentiation and activation. It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function. Helios is also involved in tumor immunity.
100μg
Verified Reactivity: Mouse, Human
Antibody Type: Recombinant
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested Block - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone QA20A52 is able to completely block the staining of clone 22F6 on target cells.
RRID: AB_2904278 (BioLegend Cat. No. 105402)
Structure: A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution: Regulatory T cells (Tregs), conventional T cells, thymocytes
Function: Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Cell Type: T cells, Thymocytes, Tregs
Biology Area: Adaptive Immunity, Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Kelly CM, et al. 1998. Curr. Biol. 8:508. Dovat S, et al. 2005. J. Immunol. 175:3508. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167. Cai Q, et al. 2009. J. Immunol. 183:2303. Zhang Z, et al. 2007. Blood 109:2190. Hahm K, et al. 1998. Genes Dev. 12:782.
Gene ID: 2277922807
UniProt: View information about Helios on UniProt.org
Clone: QA20A52
Regulatory Status: RUO
Other Names: IKZF2, IKAROS family zinc finger 2, ANF1A2, ZNF1A2, ZNFN1A2
Isotype: Rat IgG1, κ