Biolegend, 105601, Purified anti-mouse CD102 Antibody, 50μg

CD102 is a 55 kD immunoglobulin superfamily member, also known as ICAM-2. CD102 is a heavily glycosylated cell surface ligand for LFA-1 (CD11a/CD18). CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation and T cell activation. The 3C4(MIC2/4) antibody can block interactions between ICAM-2 and LFA-1.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: COS cells transfected with mouse ICAM-2 cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections, in vitro blocking of ligand binding1, and immunoprecipitation1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105608).
Application References(PubMed link indicates BioLegend citation): Xu H, et al. 1996. J. Immunol. 156:4909. Gerwin N, et al. 1999. Immunity 10:9.
Product Citations: Zuchtriegel G, et al. 2016. PLoS Biol. 14: 1002459. PubMed Osokine I, et al. 2022. Cell Rep. 38:110329. PubMed Reglero-Real N, et al. 2021. Immunity. :. PubMed Umugire A, et al. 2022. Clin Exp Otorhinolaryngol. 15:220. PubMed Saitoh S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01687-x. PubMed
RRID: AB_313194 (BioLegend Cat. No. 105601) AB_313195 (BioLegend Cat. No. 105602)
Structure: Ig superfamily, 55 kD
Distribution: Vascular endothelial cells, some resting lymphocytes, monocytes, platelets
Function: Lymphocyte recirculation, costimulation
Ligand/Receptor: LFA-1, Mac-1
Cell Type: Endothelial cells, Lymphocytes, Monocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Casasnovas JM, et al. 1999. P. Natl. Acad. Sci. USA 96:3017. 3. Diamond MS, et al. 1990. J. Cell Biol. 111:3129.
Gene ID: 15896
UniProt: View information about CD102 on UniProt.org
Clone: 3C4 (MIC2/4)
Regulatory Status: RUO
Other Names: ICAM-2
Isotype: Rat IgG2a, κ