Biolegend, 105716, PerCP/Cyanine5.5 anti-mouse CD106 Antibody, 100μg

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α 4 /β 1 integrin) and LPAM-1 (α 4 /β 7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse preadipose cell line PA6
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 of acetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo, immunoprecipitation1 , and spacial biology (IBEX)11,12. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105727 & 105728).
Application References(PubMed link indicates BioLegend citation): Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC) Kinashi T, et al. 1994. Blood Cells 20:25. (Block) Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC) Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC) Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed Monnier J, et al. 2012. J. Immunol. 189:956. PubMed Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Hum NR, et al. 2020. Cancers (Basel). :12. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Hochrainer K, et al. 2013. J Biol Chem. 288:285. PubMed Nahrendorf W, et al. 2021. eLife. 10:00. PubMed Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
RRID: AB_1595594 (BioLegend Cat. No. 105715) AB_1595489 (BioLegend Cat. No. 105716)
Structure: Ig superfamily, 47 kD
Distribution: Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells
Function: Adhesion
Ligand/Receptor: VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin)
Cell Type: Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. 3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767. 4. Koni PA, et al. 2001. J. Exp. Med. 193:741.
Gene ID: 22329
UniProt: View information about CD106 on UniProt.org
Clone: 429 (MVCAM.A)
Regulatory Status: RUO
Other Names: VCAM-1, INCAM-110
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.