Biolegend, 105826, APC/Cyanine7 anti-mouse CD117 (c-kit) Antibody, 100μg

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse bone marrow mast cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.
Application References(PubMed link indicates BioLegend citation): Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC) Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC) Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC) Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed Gómez-Salinero JM, et al. 2022. Nat Cardiovasc Res. 1:882. PubMed Sambandam A, et al. 2023. Heliyon. 9:e14238. PubMed Herrejon Chavez F, et al. 2023. Nat Commun. 14:2290. PubMed Kang YA, et al. 2023. J Exp Med. 220:. PubMed Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed Kfoury YS, et al. 2021. Cell Stem Cell. 28:2090. PubMed Wang D, et al. 2022. Cell Rep. 39:110587. PubMed Martin-Martin I, et al. 2022. Cell Rep. 39:110648. PubMed Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed Roy IM, et al. 2022. iScience. 25:105171. PubMed Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed Gonçalves R, et al. 2023. iScience. 26:105972. PubMed Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed Domingues AF, et al. 2020. Elife. 9:e51754. PubMed Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed Hu X, et al. 2016. Nat Commun. 7:13095. PubMed Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed Iturri L, et al. 2021. Immunity. . PubMed Wu X, et al. 2016. Nat Commun. 7:10533. PubMed Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed Wu HC, et al. 2021. Cancer Discov. Online ahead of print. PubMed Li Z, et al. 2022. Elife. 11:. PubMed Taylor D, et al. 2011. J Immunol. 186:5956. PubMed Nguyen DTT, et al. 2020. Nat Commun. 11:2026. PubMed Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed Li Y, et al. 2021. Nat Commun. 0.843055556. PubMed Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed Nurkhametova D, et al. 2019. Front Cell Neurosci. 13:45. PubMed Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed Yu VWC, et al. 2017. Cell. 168:944. PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Jia W, et al. 2021. Nat Commun. 12:2118. PubMed Reeh K, et al. 2014. Development. 141:2950. PubMed Beyaz S, et al. 2016. Nature. 531:53-58. PubMed Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed Bell CC, et al. 2019. Nat Commun. 10:2723. PubMed Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed Carr M, et al. 2016. Proc Natl Acad Sci U S A. 113(52):15024-15029. PubMed Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed Comoglio F, et al. 2018. Genome Res. 10.1101/gr.227272.117. PubMed Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed Minuesa G, et al. 2019. Nat Commun. 10:2691. PubMed Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed Ding P, et al. 2022. Bone Res. 10:42. PubMed Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed Nowlan B, et al. 2019. Haematologica. 105:71. PubMed Cazares O, et al. 2021. Development. 148:. PubMed Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed Mana MD, et al. 2021. Cell Reports. 35(10):109212. PubMed Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed Abdala-Valencia H, et al. 2015. J Immunol. 195: 1377-1387. PubMed Rüder C, et al. 2014. PLoS One. 9:104644. PubMed Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed Wang S, et al. 2021. Commun Biol. 22:. PubMed Bikorimana E, et al. 2014. J Vis Exp. 92: 52022. PubMed Stine RR, et al. 2020. Cell Stem Cell. 25(6):830-845. PubMed Shigeta A, et al. 2019. Dev Cell. 48:617. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Koide S, et al. 2022. iScience. 25:103603. PubMed Ortega-Molina A, et al. 2021. Cell Reports. 36(2):109372. PubMed Kassas–Guediri A, et al. 2017. PLoS One. 12:e0173815. PubMed Krivtsov AV, et al. 2020. Cancer Cell. 36(6):660-673.e11.. PubMed Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed Lee SG, et al. 2020. J Nucl Med. . PubMed Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed Ito K et al. 2019. Cell Rep. 28(10):2480-2490 . PubMed Li X, et al. 2019. Stem Cells. 37:937. PubMed Luo H, et al. 2019. Cell Rep. 26:945. PubMed Morganti C, et al. 2022. EMBO Rep. 23:e54262. PubMed Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed Chavez JS, et al. 2022. Cells. 11:. PubMed Fielding C, et al. 2022. Nat Commun. 13:543. PubMed Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed Chaudhury S, et al. 2018. Nat Commun. 9:5280. PubMed Dingler FA, et al. 2020. Mol Cell. . PubMed Young K, et al. 2021. Cell Stem Cell. . PubMed Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed Jee D et al. 2018. Molecular cell. 69(2):265-278 . PubMed
RRID: AB_1626280 (BioLegend Cat. No. 105825) AB_1626278 (BioLegend Cat. No. 105826)
Structure: Ig superfamily, 145 kD
Distribution: Hematopoietic stem cells, AML, mast cells
Function: Growth factor receptor, tyrosine kinase
Ligand/Receptor: Stem Cell Factor (SCF)
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Galli SJ, et al. 1994. Adv. Immunol. 55:1. 3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759. 4. Besmer P, et al. 1986. Nature 320:415. 5. Witte ON. 1990. Cell 63:5.
Gene ID: 16590
UniProt: View information about CD117 on UniProt.org
Clone: 2B8
Regulatory Status: RUO
Other Names: c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype: Rat IgG2b, κ