Biolegend, 105904, Biotin anti-mouse CD122 (IL-2Rβ) Antibody, 500μg

CD122 is a 70-75 kD IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. It is constitutively expressed by NK cells and at lower levels by T cells, B cells, monocytes, and macrophages. The IL-2Rβ chain can combine with either the common γ subunit (γ c , CD132) alone or with the γ c subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation. The 5H4 antibody does not block IL-2 binding to the IL-2 receptor. CD122 is expressed on murine, but not human, CD8+ Tregs involved in the maintenance of T cell homeostasis.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Rat myeloma YB2/0 transfected with truncated mouse Il-2Rβ cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1,2.
Application References(PubMed link indicates BioLegend citation): Furse R, et al. 1993 Eur. J. Immunol. 23:3181. (IP) He YW, et al. 1995. J. Immunol. 154:1596. (IP) Liqons DL, et al. 2012. J Biol Chem. 287:34386. PubMed.
Product Citations: McGee MC, et al. 2022. Bio Protoc. 12:. PubMed Yuan X, et al. 2017. Elife. 6:e29540. PubMed
RRID: AB_313226 (BioLegend Cat. No. 105903) AB_313227 (BioLegend Cat. No. 105904)
Structure: Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD
Distribution: T cells and B cells, NK cells, monocytes, macrophages
Function: Critical component of IL-2 and IL-15 signaling
Ligand/Receptor: IL-2, IL-15
Cell Type: B cells, Macrophages, Monocytes, NK cells, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245. 3. Suzuki H, et al. 1995. Science 268:1472. 4. Shi Z, et al. 2009. Eur. J. Immunol. 39:2109.
Gene ID: 16185
UniProt: View information about CD122 on UniProt.org
Clone: 5H4
Regulatory Status: RUO
Other Names: IL-2 Receptor β chain, IL-2Rβ
Isotype: Rat IgG2a, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.