Biolegend, 106311, Brilliant Violet 421™ anti-mouse CD152 Antibody, 125microl

CD152, also known as CTLA-4 or Ly-56, is a 33 kD member of the immunoglobulin superfamily. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.
125microl
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: Mouse CTLA-4-mouse IgG2a fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: The UC10-4B9 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation, in vitro and in vivo blocking1-4 of ligand binding, and as ELISA capture antibody5. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106327).
Application References(PubMed link indicates BioLegend citation): Walunas TL, et al. 1994. Immunity 1:405. (Block, IP) Cilio CM, et al. 1998. J. Exp. Med. 188:1239. (Block) Issazadeh S, et al. 1999. J. Immunol. 162:761. (Block) McCoy K, et al. 1997. J. Exp. Med. 186:183. (Block) Hsu HC, et al. 2007. J. Immunol. 178:5357. (ELISA Capture) Sugita S, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:5783. PubMed
Product Citations: Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed Zhu X, et al. 2023. Front Immunol. 14:1114802. PubMed Su W, et al. 2020. Cell Metabolism. 32(6):996-1011.e7. PubMed Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed Kurniawan H, et al. 2020. Cell Metabolism. 31(5):920-936. PubMed Singh M, et al. 2017. Nat Commun. 8:1447. PubMed Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed Pasciuto E, et al. 2020. Cell. 182:625. PubMed Tian M, et al. 2021. Elife. 10:. PubMed Mondini M, et al. 2015. Mol Cancer Ther. 14:1336. PubMed Hekim C, et al. 2017. Cancer Immunol Res. 5:157. PubMed Phadke MS, et al. 2021. Cancer Immunol Res. 9:554. PubMed
RRID: AB_10901170 (BioLegend Cat. No. 106311) AB_2563063 (BioLegend Cat. No. 106312)
Structure: Ig superfamily, 33 kD
Distribution: Activated T cells and B cells
Function: Negative regulator of T cell activation
Ligand/Receptor: CD80 (B7-1), CD86 (B7-2)
Cell Type: B cells, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Allison JP, et al. 1995. Science 270:932. 3. Waterhouse P, et al. 1995. Science 270:985. 4. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
Gene ID: 12477
UniProt: View information about CD152 on UniProt.org
Clone: UC10-4B9
Regulatory Status: RUO
Other Names: Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56
Isotype: Armenian Hamster IgG
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.