Biolegend, 107904, PE anti-mouse Podocalyxin Antibody, 100μg

Podocalyxin is sialomucin, most closely related to CD34 and endoglycan, expressed by kidney podocytes, hematopoietic progenitors, vascular endothelia, and a subset of neurons. It regulates both cell adhesion and cell morphology and plays an essential role in the formation of podocyte foot processes.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: OSM-dependent endothelial-like cell line, LO
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Onitsuka, I., et al., 2010. Gastroenterology, 138:1525-1535. Doyonnas, R., et al., 2005. Blood, 105:4170-4178. Minegishi, N., et al., 2003. Blood, 102:896-905. Minehata, K., et al., 2002. Blood, 99:2360-2368. Doyonnas, R., et al., 2001. J. Exp. Med., 194:13-28. Hara, T., et al., 1999. Immunity, 11:567-578.
RRID: AB_2922457 (BioLegend Cat. No. 107903) AB_2922457 (BioLegend Cat. No. 107904)
Structure: Single-pass type I membrane protein, sialoglycoprotein
Distribution: Apical cell membrane, membrane raft
Function: Cell adhesion, cell migration
Interaction: Forms a complex with EZR and SLC9A3R2
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology
Antigen References: Refaeli I, et al. 2020. Scientific Reports 10:9419 Economou C, et al. 2004. J Cell Sci (2004) 117 (15): 3281–94. Cait J, et al. 2019. Proc Natl Acad Sci. 116 (10) 4518-4527 Doyonnas R, et al. 2005. Blood. 105(11):4170-8 Zhang H, et al. 2014. Stem Cells. 32(1):191-203
Gene ID: 27205
UniProt: View information about Podocalyxin on UniProt.org
Clone: 10B9
Regulatory Status: RUO
Other Names: Podocalyxin
Isotype: Rat IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.