Biolegend, 109801, Purified anti-mouse CD45.2 Antibody, 50μg

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: B10.S mouse thymocytes and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested Block, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.
Application References(PubMed link indicates BioLegend citation): Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block) Yakura H, et al. 1986. J. Immunol. 136:2729. (Block) Suzuki K, et al. 2000. Immunity 13:691. (IHC) Shen FW, et al. 1986. Immunogenetics 24:146. (IP) Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837. Pascal V, et al. 2007. J. Immunol. 179:1751. Burman AC, et al. 2007. Blood 110:1064. Kincaid EZ, et al. 2007. J. Immunol. 179:3187. Phan TG, et al. 2007. Nature Immunol. 8:992. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed Wen T, et al. 2013. PNAS. 110:6067. PubMed Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations: Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed Ma J, et al. 2012. J Immunol. 188:5337. PubMed Li F, et al. 2020. EMBO J. 39:e103205. PubMed Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed Doll JR, et al. 2020. PLoS Pathog. 16:e1008296. PubMed Calcagno DM, et al. 2020. Sci Immunol. 5: . PubMed McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed Puiffe ML, et al. 2020. Front Immunol. 11:600012. PubMed Chen L, et al. 2020. Front Immunol. 11:584458. PubMed Meers GK, et al. 2018. PLoS One. 13:e0190846. PubMed Nakano-Yokomizo T, et al. 2011. J Exp Med. 208:1661. PubMed Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed Matsuba S, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01538. PubMed Lin WC, et al. 2020. J Clin Invest. 130:157. PubMed You X, et al. 2022. Front Genet. 12:790990. PubMed Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed Kolodkin-Gal D, et al. 2021. Gut. Online ahead of print. PubMed Galbas T, et al. 2017. J Immunol. 198(2):852-861. PubMed Speir M, et al. 2020. Nat Immunol. 21:54. PubMed Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed Basilico S, et al. 2020. Nat Commun. 11:1407. PubMed Lin Z, et al. 2021. Stem Cells. 39:240. PubMed Takeuchi Y, et al. 2021. Sci Rep. 11:18679. PubMed Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed Fitzpatrick Z, et al. 2020. Nature. 587:472. PubMed Udumula MP, et al. 2021. Mol Metab. 53:101272. PubMed Alam IS, et al. 2020. Cancer Res. 80:4780. PubMed
RRID: AB_313439 (BioLegend Cat. No. 109802)
Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Suzuki K, et al. 2000. Immunity 13:691.
Gene ID: 19264
UniProt: View information about CD45.2 on UniProt.org
Clone: 104
Regulatory Status: RUO
Other Names: Ly-5.2, LCA
Isotype: Mouse (SJL) IgG2a, κ