Biolegend, 110001, Purified anti-mouse I-A, 50μg

The 11-5.2 antibody reacts with the α chain of the I-A k MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 k bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 11-5.2 antibody cross-reacts with cells from mice bearing the H-2 r haplotype. Reactivity with other H-2 haplotypes ( e.g., b, d, f, p, q, s) has not been observed. The 11-5.2 antibody has been reported to block antigen presentation and induce B cell differentiation.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CKB mouse splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of MHC class II - restricted CD4+ T cell responses2,3, induction of B cell differentiation4, and immunohistochemical staining5 of acetone-fixed frozen sections. This clone does not cross react with other haplotypes (e.g., b, d, f, p, q, s).
Application References(PubMed link indicates BioLegend citation): Oi VT, et al. 1978. Curr. Top. Microbiol. Immunol. 81:115. (IP) Formby B, et al. 1990. P. Natl. Acad. Sci. USA 87:2438. Kappler JW, et al. 1981. J. Exp. Med. 153:1198. Bishop GA, et al. 1989. P. Natl. Acad. Sci. USA 86:5933. Ardehali A, et al. 1995. Circulation 92:450. (IHC) Turnquist HR, et al. 2007. J. Immunol. 178:7018. Zhang L, et al. 2011. Lupus. 20:667. PubMed.
Product Citations: Zhang L, et al. 2011. Lupus. 1.296527778. PubMed Hausmann A, et al. 2021. J Exp Med. 218:. PubMed Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed
RRID: AB_313459 (BioLegend Cat. No. 110002)
Structure: α chain of MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
Gene ID: 14960
UniProt: View information about I-Ak on UniProt.org
Clone: 11-5.2
Regulatory Status: RUO
Other Names: MHC class II
Isotype: Mouse (BALB/c) IgG2b, κ