Biolegend, 110905, APC anti-mouse CD103 Antibody, 25μg

CD103 is the alpha E integrin, which forms a heterodimer with integrin beta 7. The heterodimer complex binds to E-cadherin. It is expressed in on T cells, notably in subpopulations in the lamina propria, on regulatory T cells, or resident memory T cells, and a subset of dendritic cells in gut mucosa. CD103 is thought to be involved with homing to intestinal epithelial sites and has been associated with high levels of TGF-beta presence.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse integrin alphaE/beta7
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone W19396D can partially block binding of clone 2E7 on target cells.
RRID: AB_2927988 (BioLegend Cat. No. 110905) AB_2927988 (BioLegend Cat. No. 110906)
Structure: 125 - 150 kD transmembrane glycoprotein, integrin
Distribution: Lamina propria T cells, regulatory T cells, resident memory T cells, subset of dendritic cells
Function: Adhesion, localization and activation of intestinal lymphocytes, lymphocyte homing
Interaction: Integrin β7
Ligand/Receptor: E-Cadherin
Cell Type: Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area: Cell Adhesion, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Parker CM, et al. 1992. Proc Natl Acad Sci U S A. 89:1924-8. Kruschwitz M, et al. 1991. J Clin Pathol. 44:636-45. Schon MP, et al. 1999. J. Immunol. 162:6641-9. Shaw SK, et al. 1994. J Biol Chem. 269:6016-25.
Gene ID: 3682
UniProt: View information about CD103 on UniProt.org
Clone: W19396D
Regulatory Status: RUO
Other Names: CD103, ITAGE, integrin alpha E
Isotype: Rat IgG2b, κ