Biolegend, 111801, Purified anti-mouse CD163 Recombinant Antibody, 25μg

CD163, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen, is a member of the group B scavenger receptor cysteine-rich superfamily. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.
25μg
Verified Reactivity: Mouse
Antibody Type: Recombinant
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2936520 (BioLegend Cat. No. 111802)
Structure: Type 1 transmembrane glycoprotein, 134 kD (non-reduced)/155 kD (reduced) glycoprotein
Distribution: Macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells
Function: Binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages
Cell Type: Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Jiang L, et al. 2020. Cell. 183:1219-1233.e18. Harb H, et al. 2021. Immunity. 54:1186-1199.e7.
Gene ID: 93671
UniProt: View information about CD163 on UniProt.org
Clone: QA20A94
Regulatory Status: RUO
Other Names: M130, MM130, SCARI1
Isotype: Rat IgG1, κ