Biolegend, 112605, Spark PLUS UV395™ anti-mouse CD25 Antibody, 25μg

CD25, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac, is a 55 kD glycoprotein. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor. CD25 plays a role in lymphocyte differentiation and activation/proliferation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark PLUS UV395™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Spark PLUS UV395™ has a maximum excitation of 355 nm and a maximum emission of 385 nm.
Excitation Laser: Ultraviolet Laser (355 nm)
RRID: AB_3662172 (BioLegend Cat. No. 112605) AB_3662172 (BioLegend Cat. No. 112606)
Structure: Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution: Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
Function: IL-2 receptor
Ligand/Receptor: IL-2
Cell Type: B cells, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Taniguchi T, et al. 1993. Cell. 73:5-8. Waldmann TA. 1991. J Biol Chem. 266:2681-4. Read S, et al. 2000. J Exp Med. 192:295-302. Lowenthal JW, et al. 1985. J Immunol. 135:3988-94.
Gene ID: 16184
UniProt: View information about CD25 on UniProt.org
Clone: A18246G
Regulatory Status: RUO
Other Names: IL-2Rα, Ly-43, p55, Tac
Isotype: Rat IgG2b, κ