Biolegend, 112903, Biotin anti-mouse CD120a (TNF R Type I/p55) Antibody, 50μg

CD120a is a 55 kD Type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type I (TNFRI) or p55. It is expressed on a variety of cells at low levels. Resting T cells and erythrocytes express very little to no CD120a. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRADD and RIP, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state. The 55R-170 antibody has been shown to block in vitro and in vivo receptor signaling initiated by ligand binding.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: E. coli-expressed extracellular domain of the mouse TNFRI protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality testedELISA Detection - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Flow Cytometry: For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 112904) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).ELISA Detection1: The biotinylated 55R-170 antibody is useful as a detection antibody when paired with the Purified 55R-286 antibody (Cat. No. 113002) as the capture antibody.Additional reported applications (for the relevant formats) include:immunoprecipitation1, and In vitro and In vivo receptor blocking1. The Ultra-LEAF™Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 112905 & 112906).
Application References(PubMed link indicates BioLegend citation): Sheehan, K. C., et al. 1995. J. Exp. Med. 181:607.
RRID: AB_313528 (BioLegend Cat. No. 112903) AB_313529 (BioLegend Cat. No. 112904)
Structure: TNFR superfamily, 55 kD
Distribution: Variety of cell types at low levels
Function: Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
Ligand/Receptor: TNF-α, LT-α (TNF-β)
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Aggarwal, B. B., et al. 1985. Nature 318 665. 2. Chan, F. K.M., et al. 2000. Science 288:2351. 3. Loetscher, H., et al. 1990. Cell 61:351. 4. Rothe, J., et al. 1993. Nature 364:798.
Gene ID: 21937
UniProt: View information about CD120a on UniProt.org
Clone: 55R-170
Regulatory Status: RUO
Other Names: TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A
Isotype: Armenian Hamster IgG
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.