Biolegend, 113003, PE anti-mouse CD120a (TNF R Type I/p55) Antibody, 50μg

CD120a is a 55 kD Type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type I (TNFRI) or p55. It is expressed on a variety of cells at low levels. Resting T cells and erythrocytes express very little to no CD120a. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRADD and RIP, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: E. coli-expressed extracellular domain of the mouse TNFRI protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Flow Cytometry3,4: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113003/113004)or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).ELISA Capture2: The 55R-286 antibody is useful as a capture antibody when paired with the biotinylated 55R-170 antibody (Cat. No. 112904) as the detecting antibody.Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1.
Application References(PubMed link indicates BioLegend citation): Pinckard, J.K., et al. 1997. J. Biol Chem. 272:10784. (IP)Sheehan, K.C., et al. 1995. J. Exp. Med. 181:607. (ELISA Capture)Schlaepfer, D.D., et al. 2007. J. Biol. Chem. 282:17450. (FC)Bell, J.H., et al. 2007. J. Leukocyte Biol. 82:173. (FC)Kuroishi, T., et al. 2008. J. Leukocyte Biol. 83:912.Wang K, et al. 2012. PLoS One. 7:e52924. PubMedPereira IR, et al. 2015. PLoS Negl Trop Dis. 9:3659. PubMed
Product Citations: Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed Ma Y, et al. 2022. Mol Biol Rep. 49:3975. PubMed Pereira I, et al. 2015. PLoS Negl Trop Dis. 9:3659. PubMed Vilar-Pereira G, et al. 2016. Antimicrob Agents Chemother. 60: 4297 - 4309. PubMed Pobezinskaya EL et al. 2019. Front Immunol. 1.079861111 . PubMed Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed Li H, et al. 2018. Nat Commun. 9:2830. PubMed Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed Wang K, et al. 2012. PLoS One. 7:e52924. PubMed Puimège L, et al. 2015. EMBO Mol Med. 7: 1004-1017. PubMed Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed Akhtar MN, et al. 2020. PLoS Pathog. 16:e1009136. PubMed
RRID: AB_313532 (BioLegend Cat. No. 113003) AB_313533 (BioLegend Cat. No. 113004)
Structure: TNFR superfamily, 55 kD
Distribution: Variety of cell types at low levels
Function: Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
Ligand/Receptor: TNF-α, LT-α (TNF-β)
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Aggarwal, B. B., et al. 1985. Nature 318 665. 2. Chan, F. K.M., et al. 2000. Science 288:2351. 3. Loetscher, H., et al. 1990. Cell 61:351. 4. Rothe, J., et al. 1993. Nature 364:798.
Gene ID: 21937
UniProt: View information about CD120a on UniProt.org
Clone: 55R-286
Regulatory Status: RUO
Other Names: TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A
Isotype: Armenian Hamster IgG
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.