Biolegend, 114708, PE anti-mouse H-2K, 200μg

The 34-1-2S antibody reacts with the H-2K d /H-2D d MHC class I alloantigens expressed on nucleated cells from mice of the H-2K d /H-2D d haplotype. H-2K d /H-2D d is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 34-1-2S antibody cross-reacts with H-2K MHC class I alloantigens of the b, s, r, q, or p haplotypes.
200μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BDF Splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2, complement-mediated cytotoxicity, and immunohistochemistry of acetone-fixed frozen sections.
Application References(PubMed link indicates BioLegend citation): Ozato K, et al. 1982. Transplantation 34:113. Sester M, et al. 2000. J. Biol. Chem. 34:113.
Product Citations: van Vloten JP, et al. 2019. Mol Ther Methods Clin Dev. 13:154. PubMed Guo S, et al. 2021. Sci Rep. 11:23745. PubMed Luo N, et al. 2018. Nat Commun. 0.547222222. PubMed
RRID: AB_313607 (BioLegend Cat. No. 114708)
Structure: MHC class I
Distribution: Nucleated cells of the H-2Kd/H-2Dd haplotype
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD8
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: Watts C. 1997. Ann. Rev. Immunol. 15:821. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. York IA, et al. 1996. Ann. Rev. Immunol. 14:369.
Gene ID: 1496414972
UniProt: View information about H-2Kd/H-2Dd on UniProt.org
Clone: 34-1-2S
Regulatory Status: RUO
Other Names: MHC Class I
Isotype: Mouse (C3H) IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.