Biolegend, 115006, FITC anti-mouse I-A, 500μg

The 39-10-8 antibody reacts with the I-A d MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 d bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 39-10-8 antibody does not cross-react with other haplotypes (a, b, k, p, q, s), but has been demonstrated to cross-react with the g7 haplotype.
500μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: (C3H x BALB/c)F1 mouse cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, and immunohistochemical staining of acetone-fixed frozen sections. Does not cross-react with other haplotypes (e.g., a, b, k, p, q, s).
Application References(PubMed link indicates BioLegend citation): Hiramine C, et al. 1995. Cell. Immunol. 160:157. Wang Z, et al. 2004. J. Immunol. 172:5924. Ma XT, et al. 2006. Cancer Research 66:1169. Norian LA and Allen PM. 2004. J. Immunol. 173:835. PubMed Tian C, et al. 2007. J. Immunol. 179:6762.
Product Citations: Ohashi K, et al. 2014. Mol Biol Cell. 25:828. PubMed Meng X, et al. 2017. PLoS One. 10.1371/journal.pone.0184781. PubMed Aldon Y, et al. 2020. J Immunol. 204:903. PubMed Turner JA, et al. 2020. Immunity. 53:1202. PubMed Kodumudi KN, et al. 2019. Front Immunol. 10:1939. PubMed Ma X, et al. 2006. Cancer Res. 66:1169. PubMed Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed Kumagai S, et al. 2020. Immunity. 53(1):187-203.e8. PubMed Lisse TS, et al. 2020. NPJ Regen Med. 5:13. PubMed Wang Y, et al. 2020. Vaccines (Basel). 8:. PubMed Gupta P, et al. 2016. PLoS One. 11: 0154725. PubMed Rana MN, et al. 2021. Front Pharmacol. 12:643215. PubMed
RRID: AB_313620 (BioLegend Cat. No. 115005) AB_313621 (BioLegend Cat. No. 115006)
Structure: MHC class II
Distribution: B cell and activated T cells, APCs of H-2d mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Ann. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323. 3. Wall KA, et al. 1983. J. Immunol. 131:1056. 4. Ridgway WM, et al. 1998. J. Exp. Med. 188:2267.
Gene ID: 14961
UniProt: View information about I-Ad on UniProt.org
Clone: 39-10-8
Regulatory Status: RUO
Other Names: MHC class II
Isotype: Mouse (C3H.SW) IgG3, κ