Biolegend, 115525, Alexa Fluor® 647 anti-mouse CD19 Antibody, 25μg

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD19-expressing K562 human erythroleukemia cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality testedIHC-F - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence7.
Application References(PubMed link indicates BioLegend citation): Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC) Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed Olive V, et al. 2013. Elife. 2:822. PubMed Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations: Komuczki J, et al. 2019. Immunity. 50:1289. PubMed Ferriz M, et al. 2023. STAR Protoc. 4:102079. PubMed Mueller JPJ, et al. 2023. Front Immunol. 14:1034032. PubMed Dölz M, et al. 2022. iScience. 25:105372. PubMed Plzakova L, et al. 2014. Microb Pathog. 75:49. PubMed Barry KC, et al. 2018. Nat Med. 24:1178. PubMed Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed Gilleron J et al. 2018. Cell reports. 25(12):3329-3341 . PubMed Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed Kovács B, et al. 2020. Front Immunol. 2.508333333. PubMed Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed Krollmann C, et al. 2022. STAR Protoc. 3:101309. PubMed Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed Plzakova L, et al. 2015. PLoS One. 10: 0132571. PubMed Lindner J, et al. 2014. J Immunol. 192:4483. PubMed Sanders K, et al. 2015. Cancer Immunol Res. 3: 891-901. PubMed Gralinski L, et al. 2015. PLoS Genet. 11: e1005504. PubMed
RRID: AB_493340 (BioLegend Cat. No. 115525) AB_389329 (BioLegend Cat. No. 115522)
Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand/Receptor: CD21, CD81, Leu-13
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478
UniProt: View information about CD19 on UniProt.org
Clone: 6D5
Regulatory Status: RUO
Other Names: B4
Isotype: Rat IgG2a, κ