Biolegend, 115529, APC/Cyanine7 anti-mouse CD19 Antibody, 25μg

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD19-expressing K562 human erythroleukemia cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence7.
Application References(PubMed link indicates BioLegend citation): Shoham T, et al. 2003. J. Immunol. 171:4062. (FC) Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC) Kamimura D, et al. 2006. J. Immunol. 177:306. (FC) Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC) Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC) Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed Olive V, et al. 2013. Elife. 2:822. PubMed Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations: Laidlaw BJ, et al. 2020. Nat Immunol. 1.626388889. PubMed Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed Pérez-Torrado V, et al. 2022. Bio Protoc. 12:. PubMed Liu KY, et al. 2023. Environ Health Perspect. 131:37014. PubMed Zhang Z, et al. 2023. Aging Dis. 14:966. PubMed Lütge M, et al. 2023. Nat Immunol. . PubMed Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed Pani F, et al. 2021. Endocrinology. 162:. PubMed Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed Xin B, et al. 2022. Hepatology. 76:630. PubMed Schnell A, et al. 2021. Cell. 184:6281. PubMed Laffleur B, et al. 2022. Sci Immunol. 7:eabn2738. PubMed Grégoire C, et al. 2022. Immunity. 55:1216. PubMed Backlund C, et al. 2022. Proc Natl Acad Sci U S A. 119:e2204078119. PubMed Gonçalves R, et al. 2023. iScience. 26:105972. PubMed Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed Proietti M, et al. 2019. Nat Commun. 10:250. PubMed Ferrere G, et al. 2021. JCI Insight. 6:. PubMed Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed Machata S, et al. 2021. Front Immunol. 11:565869. PubMed Moodley D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9852 - 9857. PubMed Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed Tang J, et al. 2018. Mol Cell Biol. 38:e00103. PubMed Quatrini L, et al. 2018. Nat Immunol. 19:954. PubMed Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Chikina AS, et al. 2020. Cell. 183:411. PubMed Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed Tilstam PV, et al. 2021. J Clin Invest. 131:. PubMed Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed Trott DW, et al. 2021. Geroscience. 43:1331. PubMed Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed Inoue D, et al. 2021. Nat Genet. 53:707. PubMed Bresque M, et al. 2022. J Biol Chem. :101711. PubMed Mukherjee D, et al. 2022. Nat Commun. 13:3747. PubMed Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed Lercher A, et al. 2019. Immunity. 51:1074. PubMed Stone AEL, et al. 2019. Nat Commun. 10:3649. PubMed Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed Lee M, et al. 2014. PLoS One. 9:112666. PubMed Koh F, et al. 2015. Proc Natl Acad Sci U S A. 112:1734. PubMed Wei SC, et al. 2020. Cancer Discov. . PubMed Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed Ghilas S, et al. 2021. iScience. 24:103059. PubMed Kim P, et al. 2015. J Exp Med. 212:633. PubMed Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed Weber G, et al. 2014. J Exp Med. 211:1243. PubMed Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed Qi T, et al. 2020. Cell Rep. 33:108452. PubMed Xu J et al. 2018. Cell. 173(3):762-775 . PubMed Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed Russler-Germain EV, et al. 2021. Elife. 10:. PubMed Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed Pitsch J, et al. 2021. Ann Neurol. 89:666. PubMed Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed Zhang H, et al. 2019. Mol Cell. 76:110. PubMed Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed Hassan AO, et al. 2020. Cell. 183:169. PubMed Carbone C, et al. 2021. J Immunother Cancer. 9:. PubMed Elkhatib SK, et al. 2021. Biol Psychiatry Glob Open Sci. 1:190. PubMed Colombo M, et al. 2022. iScience. 25:105042. PubMed Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed Yahagi A, et al. 2019. RMD Open. 5:e000853. PubMed Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed Fischer FA, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed Trott DW, et al. 2021. J Physiol. 599:3973. PubMed Engblom C, et al. 2017. Science. 358:6367. PubMed Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed Barbet G, et al. 2018. Immunity. 48:584. PubMed Urata S, et al. 2018. PLoS Pathog. 14:e1007172. PubMed Liu YG, et al. 2019. FASEB J. 33:5018. PubMed Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed Du P, et al. 2022. Front Pharmacol. 13:805508. PubMed Strawbridge R, et al. 2016. Diabetes. 65: 2888 - 2899. PubMed Yoshida H, et al. 2019. Cell. 176:897. PubMed La Salvia S, et al. 2020. Am J Physiol Renal Physiol. 319:F868. PubMed Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed Chavez JS, et al. 2022. Cells. 11:. PubMed Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed Wilson CS, et al. 2021. JCI Insight. 6:. PubMed Rivera CA, et al. 2022. Immunity. 55:129. PubMed Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed Cheng Q, et al. 2020. Eur J Immunol. 50:284. PubMed
RRID: AB_830706 (BioLegend Cat. No. 115529) AB_830707 (BioLegend Cat. No. 115530)
Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand/Receptor: CD21, CD81, Leu-13
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF, et al. 1994. Immunol. Today 15:437.
Gene ID: 12478
UniProt: View information about CD19 on UniProt.org
Clone: 6D5
Regulatory Status: RUO
Other Names: B4
Isotype: Rat IgG2a, κ