Biolegend, 116424, APC/Fire™ 750 anti-mouse I-A, 100μg

The AF6-120.1 antibody reacts with the I-A b MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 b bearing mice, and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The AF6-120.1 antibody cross-reacts with H-2 k and H-2 u haplotypes; this antibody does not cross-react with other haplotypes (d, f, q, r, s).
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C57BL/10J splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of frozen sections (acetone-fixed5; OCT-embedded, ethanol-fixed sections7), immunofluorescence microscopy3 (including acetone-fixed epidermal sheets6), immunoprecipitation7,8. Directly conjugated antibody was used for IF in (3) and (6) and for IHC in (5). Does not react with other haplotypes (e.g., d, f, q, r, s).
Application References(PubMed link indicates BioLegend citation): Wall KA, et al. 1983. J. Immunol. 131:1056. (FC) Cohn LE, et al. 1986. P. Natl. Acad. Sci. USA 83:747. (FC) Inaba K, et al. 1998. J. Exp. Med. 188:2163 (IF) Hamrah P, et al. 2002. Invest Opthalmol Vis. Sci. 43:639 (IF) Buono C, et al. 2003. Arterioscler. Thromb. Vasc. Biol. 23:454. (IHC) Wang Z, et al. 2004. J. Immunol. 172:5924. (IHC IF) Nakagawa TY, et al. 1999. Immunity 10:207. (IP) Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC IP) PubMed Schneppenheim J, et al. 2013. J Exp Med. 210:41. PubMed.
RRID: AB_2721486 (BioLegend Cat. No. 116423) AB_2721487 (BioLegend Cat. No. 116424)
Structure: MHC class II
Distribution: B cell and activated T cells, APCs of H-2b mice
Function: Antigen presentation
Ligand/Receptor: CD3/TCR, CD4
Cell Type: Antigen-presenting cells, B cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Watts C. 1997. Annu. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323.
Gene ID: 14961
UniProt: View information about I-Ab on UniProt.org
Clone: AF6-120.1
Regulatory Status: RUO
Other Names: MHC class II
Isotype: Mouse (BALB/c) IgG2a, κ