Biolegend, 117203, PE anti-mouse CD73 Antibody, 25μg

CD73 (ecto-5'-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: BALB/c mouse splenocytes and CHO cells transfected with the mouse CD73 gene, Nt5
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The TY/23 antibody has been reported to inhibit the enzymatic activity of CD73.
Application References(PubMed link indicates BioLegend citation): 1. Zhang B. 2010. Cancer Res. 70:6407-11. 2. Stagg J, et al. 2010. Proc Natl Acad Sci U S A. 107:1547-52. 3. Forte G, et al. 2012. J Immunol. 189:2226-33.
RRID: AB_3068054 (BioLegend Cat. No. 117203) AB_3068054 (BioLegend Cat. No. 117204)
Structure: 69 kD GPI-anchored surface protein
Distribution: Positive on most CD11b+ myeloid cells in bone marrow, but not on CD11b+ peripheral cells; a large proportion of CD4+ and CD8+ splenic T cells express CD73
Function: A nucleotidase catalyzing the dephosphorylation of extracellular nucleoside 5' monophosphates
Ligand/Receptor: Nucleoside 5' monophosphate
Cell Type: B cells, Mesenchymal Stem Cells, Tregs
Biology Area: Costimulatory Molecules, Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: Yamashita Y, et al. 1998. Eur J Immunol. 28:2981-90. Resta R, et al. 1998. Immunol Rev. 161:95-109.
Gene ID: 23959
UniProt: View information about CD73 on UniProt.org
Clone: TY/23
Regulatory Status: RUO
Other Names: 5'-nucleotidase, Nt5e, 5'-NT, Ecto-5'-nucleotidase
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.