Biolegend, 117405, PE anti-mouse CD278 (ICOS) Antibody, 50μg

The 7E.17G9 antibody reacts with the 47-57 kD ICOS protein, also known as inducible costimulatory molecule, and H4. This protein is homologous to the CD28/CTLA-4 proteins. ICOS is expressed on activated T cells and a subset of thymocytes and can costimulate T cells and induce proliferation. In addition ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand, B7h/B7RP-1 and B7-H2 is constitutively expressed in B cell areas of secondary lymphoid organs and can be induced in other tissues by LPS. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. ICOS stimulation has been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse ICOS cDNA and ICOS hexahistidine fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: blocking of ligand binding. The LEAF™ format is suggested for blocking studies.
Application References(PubMed link indicates BioLegend citation): Akbari O, et al. 2002. Nat. Med. 8:1024. Harada H, et al. 2003. J. Clin. Invest. 112:234. McAdam AJ, et al. 2000. J. Immunol. 165:5035. (FC Block) Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
Product Citations: Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed Bajaj R, et al. 2022. Cell Rep. 40:111429. PubMed Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed Tan X, et al. 2021. Sci Adv. 7: . PubMed Yu X, et al. 2019. Nat Commun. 10:574. PubMed Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed Marcandalli J et al. 2019. Cell. 176(6):1420-1431 . PubMed Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
RRID: AB_961244 (BioLegend Cat. No. 117405) AB_2122712 (BioLegend Cat. No. 117406)
Structure: CD28/CTLA-4, 47-57kD
Distribution: Activated T cells, subset of thymocytes
Function: Costimulates T cell activation, proliferation, humoral immune response
Ligand/Receptor: B7h/B7RP-1/GL-50
Cell Type: T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544. 2. McAdam AJ, et al. 2000. J. Immunol. 165:5035. 3. Mak TW, et al. 2003. Nat. Immunol. 4:765
Gene ID: 54167
UniProt: View information about CD278 on UniProt.org
Clone: 7E.17G9
Regulatory Status: RUO
Other Names: Inducible COStimulatory molecule, H4
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.